LOCUS       sequence-121257-         655 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for tdTomato knock-in via landing pad.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, 
            Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB
  TITLE     One-step generation of multiple transgenic mouse lines using an 
            improved Pronuclear Injection-based Targeted Transgenesis (i-PITT).
  JOURNAL   BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5.
  PUBMED    25887549
REFERENCE   2  (bases 1 to 655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2015-04-9"; volume: "16:274. doi"; pages: " 
            10.1186/s12864-015-1432-5"
COMMENT     Sequence Label: pBEZ
FEATURES             Location/Qualifiers
     source          1..655
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    107..140
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    160..193
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    213..246
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    266..299
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    319..352
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    372..405
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     regulatory      473..482
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(612..630)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 aggtgagcca gtgagttgat tgcagtccag ttacgctgga gtctgaggct cgtcctgaat
       61 gatatgcggc ctcggatccg cgatcgatcc gctagccgcg gtgcgggtgc cagggcgtgc
      121 ccttgggctc cccgggcgcg tactccaccc gcggtgcggg tgccagggcg tgcccttggg
      181 ctccccgggc gcgtactcca cccgcggtgc gggtgccagg gcgtgccctt gggctccccg
      241 ggcgcgtact ccacccgcgg tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt
      301 actccacccg cggtgcgggt gccagggcgt gcccttgggc tccccgggcg cgtactccac
      361 ccgcggtgcg ggtgccaggg cgtgcccttg ggctccccgg gcgcgtactc cacctaggaa
      421 gttcctattc cgaagttcct attctatcag aagtatagga acttcaccgg tcgccaccat
      481 ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg
      541 ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
      601 cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgg
//
