LOCUS       sequence-121260-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETDuet-1 Containing FadD V4F, W5L -His.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Ford TJ, Way JC
  TITLE     Enhancement of E. coli acyl-CoA synthetase FadD activity on medium 
            chain fatty acids.
  JOURNAL   PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 
            2015.
  PUBMED    26157619
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2015-06-30"; volume: "3:e1040. doi"; pages: " 
            10.7717/peerj.1040. eCollection 2015"
COMMENT     Sequence Label: PAS531 (TJF074)
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..62)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    171..195
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(195..214)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(196..214)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(303..320)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tatagctgcg ctgtagacga gtccatgtgc tggcgttcaa atttcgcagc agcggtttct
       61 ttaccanact cgagggtacc gacgtcagcg atcgcgtggc cggccgatat ccaattgaga
      121 tctgccatat gtatatctcc ttcttatact taactaatat actaagatgg ggaattgtta
      181 tccgctcaca attcccctat agtgagtcgt attaatttcg attatgcggc cgtgtacaat
      241 acgattactt tctgttcgac ttaagcatta tgcggccgca agcttggatc ctcattcatt
      301 cagtgatgat gatggtgatg agaagaaccg gctttattgt ccactttgcc gcgcgcttcg
      361 tcacgtaatt ctcgtcgcaa aatttttccg acgttagatt tcggtaactc atcacgaaac
      421 tccaccagct tcggtacttt gtagcccgtg agctgacggc ggcaaaaggt caccagtgac
      481 tcttcggtaa gcgatggatc ttttttcact acgaagattt tcaccgcttc accactggag
      541 ccggaaggta cgccaacagc cgcgacttcc tgtacgccag gatgctgcat gacgacatct
      601 tcaatctcgt tgggatagac gttaaaaccg gaaaccagaa tcatgtcttt tttacgatcg
      661 acaatgcgca ggaacccttc ttcatccatc accgcgatgt cgccggtgtg taaccagcca
      721 tttttgatga tctcatctgt agcatccgga cgctgccagt aacccagcat cacctgcggt
      781 cctttgacac aaagctcacc cggttgaccc ggtggtactt cattatcatc atcatccacc
      841 agtttggctt ccgtcgacgg caccggcaaa ccgatgctac cactatgata atcaatatca
      901 tatgggttaa cgctgaccag cggcgcacac tcggtaaggc catagccttc cagcagatac
      961 tgtcctgtca gtttcaccca acgctctgcc acc
//
