LOCUS       sequence-121262-         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETDuet-1 Containing WT FadD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Ford TJ, Way JC
  TITLE     Enhancement of E. coli acyl-CoA synthetase FadD activity on medium 
            chain fatty acids.
  JOURNAL   PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 
            2015.
  PUBMED    26157619
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2015-06-30"; volume: "3:e1040. doi"; pages: " 
            10.7717/peerj.1040. eCollection 2015"
COMMENT     Sequence Label: PAS500 (TJF032)
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..66)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    175..199
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(199..218)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(200..218)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(307..324)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cnggtatagc tgcgctgtag acgagtccat gtgctggcgt tcaaatttcg cagcagcggt
       61 ttctttacca gactcgaggg taccgacgtc agcgatcgcg tggccggccg atatccaatt
      121 gagatctgcc atatgtatat ctccttctta tacttaacta atatactaag atggggaatt
      181 gttatccgct cacaattccc ctatagtgag tcgtattaat ttcgattatg cggccgtgta
      241 caatacgatt actttctgtt cgacttaagc attatgcggc cgcaagcttg gatcctcatt
      301 cattcagtga tgatgatggt gatgagaaga acctcaggct ttattgtcca ctttgccgcg
      361 cgcttcgtca cgtaattctc gtcgcaaaat ttttccgacg ttagatttcg gtaactcatc
      421 acgaaactcc accagcttcg gtactttgta gcccgtgagc tgacggcggc aaaaggtcac
      481 cagtgactct tcggtaagcg atggatcttt tttcactacg aagattttca ccgcttcacc
      541 actggagccg gaaggtacgc caacagccgc gacttcctgt acgccaggat gctgcatgac
      601 gacatcttca atctcgttgg gatagacgtt aaaaccggaa accagaatca tgtctttttt
      661 acgatcgaca atgcgcagga acccttcttc atccatcacc gcgatgtcgc cggtgtgtaa
      721 ccagccattt ttgatgatct catctgtagc atccggacgc tgccagtaac ccagcatcac
      781 ctgcggtcct ttgacacaaa gctcacccgg ttgacccggt ggtacttcat tatcatcatc
      841 atccaccagt ttggcttccg tcgacggcac cggcaaaccg atgctaccac tatgataatc
      901 aatatcatat gggttaacgc tga
//
