LOCUS       sequence-121270-         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mobilizible plasmid with EGFP. OriT from the F plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Leonard J
  TITLE     Leonard Lab Conjugation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pShuttle
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..21
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(25..68)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    71..92
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    93..111
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    118..136
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             155..166
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             173..886
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(203..231)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     826..846
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg
       61 atgatttctg gaattcgcgg ccgcttctag agtccctatc agtgatagag attgacatcc
      121 ctatcagtga tagagatact gagcactact agagaaagag gagaaatact agatgcgtaa
      181 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa
      241 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac
      301 ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac
      361 tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt
      421 tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga
      481 cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat
      541 cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata
      601 caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt
      661 taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag accattatca
      721 acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac
      781 acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt
      841 tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat aatactagag
      901 ccaggcatca a
//
