LOCUS       sequence-121274-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mobilizible plasmid with EGFP. OriT from the F plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Leonard J
  TITLE     Leonard Lab Conjugation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pShuttle
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     203..220
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(244..301)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    302..322
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      complement(329..356)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(372..443)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(506..526)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctgtggaaaa acgccagcaa
       61 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      121 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      181 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcctgca taacgcgaag
      241 taatcttttc ggttttaaag aaaaagggca gggtggtgac accttgccct tttttgccgg
      301 actgcagcgg ccgctactag tatataaacg cagaaaggcc cacccgaagg tgagccagtg
      361 tgactctagt agagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc
      421 gactgagcct ttcgttttat ttgatgcctg gctctagtat tattatttgt atagttcatc
      481 catgccatgt gtaatcccag cagctgttac aaactcaaga aggaccatgt ggtctctctt
      541 ttcgttggga tctttcgaaa gggcagattg tgtggacagg taatggttgt ctggtaaaag
      601 gacagggcca tcgccaattg gagtattttg ttgataatgg tctgctagtt gaacgcttcc
      661 atcttcaatg ttgtgtctaa ttttgaagtt aactttgatt ccattctttt gtttgtctgc
      721 catgatgtat acattgtgtg agttatagtt gtattccaat ttgtgtccaa gaatgtttcc
      781 atcttcttta aaatcaatac cttttaactc gattctatta acaagggtat caccttcaaa
      841 cttgacttca gcacgtgtct tgtagttccc gtcatctttg aaaaatatag ttctttcctg
      901 tacataacct tcggg
//
