LOCUS       sequence-121283-         183 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express soluble His-tagged zebrafish thrombopoietin in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 183)
  AUTHORS   Svoboda O, Stachura DL, Machonova O, Pajer P, Brynda J, Zon LI, 
            Traver D, Bartunek P
  TITLE     Dissection of vertebrate hematopoiesis using zebrafish 
            thrombopoietin.
  JOURNAL   Blood. 2014 Jul 10;124(2):220-8. doi: 10.1182/blood-2014-03-564682. 
            Epub 2014 May 28.
  PUBMED    24869937
REFERENCE   2  (bases 1 to 183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/blood-2014-03-564682"; journalName: "Blood"; date: 
            "2014-07-10- 10"; volume: "124"; issue: "2"; pages: "220-8"
COMMENT     Sequence Label: pAc-­His-­zfTpo
FEATURES             Location/Qualifiers
     source          1..183
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     5..64
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     CDS             89..106
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 caagatggta agcgctattg ttttatatgt gcttttggcg gcggcggcgc attctgcctt
       61 tgcggcggat cttggatcta tggctagcca ccatcaccat caccatggtg gatccactag
      121 accaattgac tttgtgtgtg acagcgaggc caggagagtg atgaacaagg tgaaggatct
      181 aca
//
