LOCUS       sequence-121291-       11137 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High copy number plasmid encoding part of the tra Operon traC-artA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11137)
  AUTHORS   Leonard J
  TITLE     Leonard Lab Conjugation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLNKR2
FEATURES             Location/Qualifiers
     source          1..11137
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(11..54)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      9082..9139
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(9163..9180)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9334..9922)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9414..9433)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10093..10884,10885..10953))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10716..10735
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10954..11058)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(11126..11137,1..7)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat
       61 tcgcggccgc accagttagg cgcgccatct acgttccgcg gttccggatg aacatgagtg
      121 gatattcaga aaacagaaat ctttgtatat gcgccgggaa atggcgcgtt aattacaggt
      181 attcccttca tggctaccgc atttctcgct ttatttttca actaaggaat tcatgtgaat
      241 aacccacttg aggccgtcac tcaggcggtt aactccctcg tcacagcact gaaactgcct
      301 gacgaatccg caaaggccaa tgaagttctg ggcgaaatga gcttcccgca gttcagccgt
      361 ctgctgccgt accgtgatta caaccaggaa tccggtctgt tcatgaatga caccacgatg
      421 ggctttatgc tggaagccat tcccatcaat ggggcgaatg agtccattgt ggaggccctc
      481 gatcatatgc tgcgcaccaa actgccgcgc ggtattccgt tgtgtatcca tctgatgtcc
      541 agtcagctgg ttggtgacag gattgaatac gggctgcgtg agttctcctg gtctggcgaa
      601 caggccgaac ggtttaacgc gattacccgg gcctattata tgaaagcggc agcgacacag
      661 tttccgctgc cggagggaat gaatctgccc ctgaccctgc gccattaccg ggtgtttatc
      721 tcgtactgtt ctccctcgaa gaaaaaaagc cgggccgaca ttctggaaat ggaaaacctg
      781 gtgaaaatca tccgggcgtc gttacagggg gccagtatca ccacacagac ggtggatgca
      841 caggccttta tcgatattgt cggggagatg attaaccata acccggattc cctgtacccg
      901 aaaagacgtc agctggaccc gtattctgat ctgaattatc agtgtgtgga ggacagtttt
      961 gatctgaaag ttcgggctga ttacctgaca ctgggcctgc gtgagaacgg caggaacagc
     1021 acggcccgca tcctgaattt ccatctggcc cgtaacccgg aaatcgcctt cctgtggaac
     1081 atggccgaca actacagcaa cctgctgaac ccggaactgt ccatctcctg tccgttcatc
     1141 ctgacgctga ctctggtggt ggaagaccag gtgaaaaccc acagcgaagc caacctgaag
     1201 tacatggacc tggagaaaaa gtcgaagacc tcctatgcca aatggtttcc gtccgtggag
     1261 aaagaggcga aggagtgggg ggaactgcgt cagcggctgg gctccggtca gtcctctgtc
     1321 gtgtcctact tcctcaacat cacagccttc tgcaaggaca ataatgaaac ggcactggaa
     1381 gtggagcagg acatcctgaa cagcttccgt aaaaacggtt ttgagctgat ttcaccgcgc
     1441 tttaaccaca tgcgcaattt cctgacctgt ctgcccttta tggccgggaa agggttgttt
     1501 aaacagctga aagaggccgg agtggtacag cgcgcagaga gctttaatgt ggccaacctg
     1561 atgccgttag tggcggataa ccccctgaca ccggcaggtc tgctggcacc cacctaccgt
     1621 aaccagctgg cgtttatcga tattttcttc cgggggatga ataacaccaa ctataacatg
     1681 gcggtctgtg gcacctccgg ggccggtaaa accgggctga tacagccact tatccgcagc
     1741 gtgctggact ccggaggctt tgccgtggtg ttcgacatgg gggatggata caagtccctg
     1801 tgtgagaaca tgggcggggt gtatctggac ggtgaaaccc tgcgttttaa cccgtttgcg
     1861 aacatcaccg atattgacca gtcagcggag cgtgtccgtg accagttgtc agtgatggcc
     1921 agccccaacg gtaacctgga tgaagtgcat gaaggtctgc tgttgcaggc ggtcagagcc
     1981 tcctggctgg ccaaagagaa cagagcacgt attgatgacg tggtggattt cctgaaaaac
     2041 gccagtgaca gcgagcagta tgccgagtca ccgactatcc gcagccgtct ggacgaaatg
     2101 attgtgctgc ttgaccagta cactgccaac ggcacttacg gccagtattt taactctgat
     2161 gagccgtccc tgcgggatga cgcaaaaatg gtggtgctgg agctgggcgg actggaagac
     2221 cgtccgtcac tgctggttgc ggtgatgttc tccctgatta tctacatcga gaacaggatg
     2281 taccgcacgc cgcgtaacct caagaaactg aacgttattg atgaaggctg gcgtctgctg
     2341 gacttcaaaa atcacaaggt cggtgaattt attgagaaag gctaccgtac ggcccgccgt
     2401 cataccggtg cctatatcac catcacacag aacatcgtcg actttgactc tgataaggcc
     2461 tccagtgctg cccgcgcggc atggggtaac tcctcctaca aaattatcct caaacagagt
     2521 gcgaaggagt tcgcgaaata caaccaactg tatccggacc agttcctgcc actgcagcgc
     2581 gacatgattg gtaagtttgg tgcggccaaa gaccagtggt tcagttcctt cctgctgcag
     2641 gtggaaaacc attcctcctg gcaccgtctg tttgtggacc cgttaagccg cgccatgtac
     2701 agctctgacg gcccggattt tgagtttgtg cagcagaagc gtaaagaggg gctgagtatt
     2761 catgaggcag tgtggcagct ggcgtggaag aagtcagggc cggaaatggc ttcgctggaa
     2821 gcctggctgg aagaacatga gaaatacagg agtgtggcat gagttcaacg cagaaacccg
     2881 ccgacgtcac ggcagaacga cgcagccact ggtggtggac ggtgccggga tgcctggcaa
     2941 tggtgttact gaacgcagcc gtcagttacg gcattgtcag actgaacgca ccggtgactg
     3001 tcgccttcaa catgaaacag accgtggatg cgttttttga cagcgccagt cagaaacaac
     3061 tgtcagaagc gcagtcaaaa gccctttcag cgcgttttaa cacggcactg gaagccagcc
     3121 tgcaggcgtg gcagcagaaa caccacgcgg tcattctggt gtcgcctgct gtggtacagg
     3181 gggcaccgga tatcacccgg gaaatccagc aggatattgc ccggcgaatg agggcggaac
     3241 catgagatgc cgggggctga ttgccctgct gatatggggg cagagtgtgg ccgccgccga
     3301 tcttggtacc tggggcgatc tgtggccggt aaaggagccg gacatgctga cggtgatcat
     3361 gcagcgcctg acggcactgg agcagtccgg tgagatgggc cggaaaatgg atgcctttaa
     3421 ggagcgggtg atccgcaaca gtctgcggcc ccctgccgtg cccggtatcg gacgcacgga
     3481 gaaatacggc agccggttgt ttgacccgtc cgtcagactg gctgcggata tccgggataa
     3541 cgaagggcgg gtgtttgccc gtcagggtga agtgatgaat ccgctgcagt atgttccttt
     3601 taaccagacg ctgtatttca tcaatggcga tgatccggcg caggtggcct ggatgaaacg
     3661 ccagacgccg cccacactgg agagcaaaat tatcctcgtg cagggcagta tcccggagat
     3721 gcagaagtct ctggacagcc gtgtttactt tgaccagaac ggtgtgctct gccagcgcct
     3781 cggcattgat caggtcccgg cacgggtgag cgccgttccc ggcgatcgct tcctgaaggt
     3841 ggaatttatt ccggcagagg agggcagaaa atgaagcgaa ggctgtggct gctgatgtta
     3901 ttccttttcg ccggtcatgt ccctgcggcg tctgcggatt ctgcctgtga ggggcgtttt
     3961 gtaaacccga tcacagatat ctgctggagc tgtattttcc cgctctcgct gggcagtatc
     4021 aaagtcagtc agggcaaggt ccccgacacg gcgaacccgt cgatgcccat tcagatttgt
     4081 ccggcaccgc cgccgctgtt caggcgtatc gggctggcca ttggttactg ggagccgatg
     4141 gcgttgacgg acgtcacccg gtcaccggga tgcatggtga acctgggctt cagcctgccg
     4201 gcttttggta aaacggcaca gggaacggcg aaaaaggatg agaagcaggt aaatggggcg
     4261 ttctatcacg ttcactggta caaatacccg ctgacgtact ggctgaacat catcacatcg
     4321 ctgggctgtc tggaaggtgg tgacatggat atcgcttatc tttctgaaat cgaccccacc
     4381 tggacggaca gcagcctgac caccattctc aatccggaag ctgtcatctt tgccaatccg
     4441 atagcacagg gagcctgcgc agcagatgcg attgccagcg cctttaatat gcctctcgat
     4501 gttctgttct ggtgtgccgg ttcgcaggga agtatgtacc cgttcaatgg ctgggtgagt
     4561 aatgagtcca gtccgttgca gtcctccctg ctggtcagtg aacgcatggc gttcaagctg
     4621 caccgtcagg gcatgattat ggaaaccatc gggaaaaata acgccgtctg taatgaatat
     4681 ccgtccccaa tcctgcccaa agaacgctgg cgttaccaga tggtgaatat gtatccggac
     4741 agcgggcagt gccacccgtt cgggcgcagc gtgacccgct gggaaaccgg gaaaaatccg
     4801 cccaacacaa agaaaaactt cggctacctg atgtggcgta aacgtaactg tgtcttcctg
     4861 tgaggtgaat gatgaagctg agtatgaaat ctctggcagc actgctgatg atgctgaacg
     4921 gggcggttat ggcgtcagaa aacgtgaaca ctcctgaaaa ccgccagttc ctgaagcagc
     4981 aggaaaattt aagcagacaa ctgcgtgaaa aacctgacca tcagctgaaa gcctgggcgg
     5041 agaaacaggt gctggaaaac ccccttcagc gttcagataa ccatttcctg gatgagctgg
     5101 ttcgtaaaca gcaggcttcg caggacggga aaccccggca gggtgctctg tattttgtgt
     5161 cgttttccat tcccgaagag gggctgaaac gaatgctggg cgaaacccgg cacttcggta
     5221 ttccggccac actgcggggc atggtgaaca atgacctgaa gaccaccgct gaagctgtgt
     5281 tgtccctggt gaaagacggc gcgactgatg gtgttcagat cgacccgacg ctgttttcac
     5341 agtacggcat tcgcactgta ccggcactgg tggtgttctg cagtcaggga tacgacatca
     5401 tccggggaaa cctgcgggtc ggccaggcgc tggagaaagt ggccgccacg ggtgactgcc
     5461 ggcaggtggc tcatgattta ctggcaggga aaggagattc cgggaaatga aacgtatttt
     5521 acctctgata ctggctctgg ttgccggcat ggcacaggct gacagcaaca gtgattaccg
     5581 ggccggctct gattttgctc accagatcaa aggacaggga agcagcagta ttcagggctt
     5641 taagccacag gagagtatcc ccggctataa cgcgaatccg gacgagacaa aatactacgg
     5701 tggcgtgact gccggcgggg atggcggcct gaaaaatgac ggcaccacgg aatgggcgac
     5761 cggtgaaacc ggaaaaacca tcacagagtc cttcatgaac aagccgaaag acattctttc
     5821 accggatgca ccgtttatcc agaccggcag ggatgtggtg aaccgggctg acagcattgt
     5881 gggaaacacc gggcagcagt gcagtgcgca ggagattagc cggagtgaat acacgaatta
     5941 cacctgtgag cgggatttac aggtggagca gtactgcaca cgaactgccc gtatggagct
     6001 gcaggggagt accacatggg aaacccggac gctggagtat gaaatgagtc agctacctgc
     6061 acgtgaagtg aatggtcagt atgttgtctc cattacctct cccgttaccg gtgaaattgt
     6121 cgatgcgcat tacagctgga gtcgtactta cctgcagaag agtgtaccta tgacaattac
     6181 agtactggga accccactga gctggaatgc caaatactca gcagatgcct catttacacc
     6241 agtacagaaa acactgacgg ccggtgttgc ttttacgtca tctcaccccg tccgggtcgg
     6301 gaatacaaaa ttcaaacgtc atacggcaat gaagctgcgt ctggttgtca gggtaaaaaa
     6361 agcctcgtac accccgtatg ttgtctggtc tgaaagctgc ccgttcagca aggagctggg
     6421 gaaactgaca aaaacagaat gtacggaggc tggcgggaac aggacgctgg tgaaagacgg
     6481 tcagtcatac agcatgtacc agagctgctg ggcataccgg gacacgtatg tcacacagtc
     6541 agcagacaag ggaacctgtc agacctacac cgacaatcct gcctgtaccc tggtgtcaca
     6601 ccagtgcgcc ttttactccg aagaaggtgc ctgtctgcat gaatatgcca cgtactcctg
     6661 tgagtcaaaa acatccggga aagtgatggt ctgtggggga gacgtcttct gtctcgatgg
     6721 cgaatgcgat aaggctcaga gcgggaagag taatgatttt gccgaagccg tttctcagct
     6781 ggcggcactg gccgcagcag gtaaggatgt tgcggcgctg aacggggtgg atgtccgtgc
     6841 tttcaccggt caggcgaaat tttgtaagaa ggctgcagcc ggatacagca actgctgtaa
     6901 ggacagcggc tggggacagg atatcgggct ggccaaatgc agcagtgatg aaaaagccct
     6961 ggctaaagca aaatcaaaca aactgaccgt cagcgtcggg gagttctgtt cgaagaaagt
     7021 gctgggtgtc tgtctggaga aaaaacgcag ctactgtcag tttgattcga agctggcgca
     7081 gatagtccag cagcaggggc gcaacggtca gttgcgtatc agttttggta gtgcgaagca
     7141 tcctgactgt cgggggatta cggttgatga gctgcagaaa attcagttca acagactgga
     7201 tttcactaac ttctacgaag acctgatgaa taaccagaaa atcccggaca gtggcgttct
     7261 gacgcagaaa gtgaaagagc agattgctga ccagctgaaa caggcaggac agtaaggtat
     7321 gggaaataac cgggggaaat gatgaaagtc atattcacgt cgaaccgttt tattgatttt
     7381 cttatccggc ttttgattac agcgattgtc atttctccgg taattatctg gtcgtgggat
     7441 acggtaaaag aaaccacggc agacgggatg cttgccgctg ctttcgttat cctttactcc
     7501 ggtgtgctcc tgtttattct gtatttttgt ttttctgccc tgactgacct gcagaaacct
     7561 gatgaaagaa aaagtgatga aaggaatgaa gatgaataaa gcattactgc cactgttact
     7621 ctgctgcttt atttttccgg cgtcaggaaa agatgcaggc tggcagtggt ataacgagaa
     7681 aataaatccg aaggaaaaag aaaataaacc tgtacctgca gccccccgtc aggaaccgga
     7741 tattatgcag aaactggccg cactgcagac ggcaacaaag cgggcgctgt acgaagccat
     7801 tctgtatccc ggcgtggata attttgtgaa atatttccgg ctgcaaaatt actgggctca
     7861 gcaggccggg cttttcacca tgagcgccag aaaggccatg ctggcacatc ctgaactgga
     7921 ctataacctg cagtacagtc attacaacgg cacggtacgg aaccagctgg cagcagacca
     7981 ggcgcagcag cgacaggcca ttgcgaaact ggctgaacac tacggcatca tgttttttta
     8041 ccgggggcag gaccccatcg acgggcaact ggcgcaggtc attaatggct tccgggatac
     8101 gtatggtctg agtgttattc ccgtttccgt ggatggcgtg attaatccgc tgttgccgga
     8161 ttcccggact gaccaggggc aggcgcagcg cctcggcgtg aaatatttcc cggccatgat
     8221 gctggttgac ccgaaacagg gcagtgttcg cccgttatca tacggcttta tttcgcagga
     8281 cgacctggca aaacagttcc tgaacgtttc tgaagatttt aaacccaatt tttaatcgcg
     8341 gatttgattt atgagtgaag attatttgaa aatgtttaca ggtgttgttc tgttaatatt
     8401 tgtcattatt gccggttatt tcttttctga gcgtaatgac aggaaaatgt ttctcctgag
     8461 ctcactggtt ttccttgttg ttaatatcgc gtgtttatat gtgcttaccg ccagtctctg
     8521 gtttctgtgt ggtgcaatta tgagtcaggg cgcagcactg gttgtttcaa tagttgcggc
     8581 cgcattaccg gacgttacga gcttcgacag gttcagaaga atatttatct gtattatgtt
     8641 gtcatcggta tggtccggag tgatgtggtt ttttataagg gggcttatga caggctaagt
     8701 catataaaac ggtcataaaa accatacaaa ataatgaggt tgattactga agaccatatg
     8761 ataaaccata ccgcaatcca gtggcttctg aatagctcac aaaaataaat atagataatt
     8821 gaaaataacc ataatacaac gatgcctgct gcgatactta ataaacttat tacaagatag
     8881 tcagggaaag cattgaccgg taatgaatgt gatgctgcat atatcagcgc aataattgcc
     8941 gcatttccca gtaacgactc tcttattatg tttctgatta tttccagttt ttctttaaaa
     9001 gatcgctttt ccatttgtac ctcgctagca cattccagta agcttctggc aatggatccc
     9061 ttcgattggc ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt
     9121 ttcttaaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct
     9181 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     9241 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     9301 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     9361 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     9421 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     9481 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     9541 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     9601 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     9661 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     9721 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     9781 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     9841 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     9901 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     9961 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    10021 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    10081 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    10141 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    10201 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    10261 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    10321 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    10381 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    10441 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    10501 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    10561 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    10621 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    10681 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    10741 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    10801 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    10861 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    10921 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    10981 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    11041 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
    11101 tattatcatg acattaacct ataaaaatag gcgtatc
//
