LOCUS       sequence-121301-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl14 with C-term Myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDSHA-ARL14-Myc
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..49)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    63..87
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(682..706)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(817..835)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 attaaggtac cgggccctac agatcctctt cagagatgag tttctgctcc atctcgaatc
       61 gaaccacttt gtacaagaaa gctgggtcgt tctgcttgaa gaacgccaaa gtgtctcctc
      121 ttgatttcat gtggctcttc acaaatccag ttaatttcct gaacccctgg gccagcccct
      181 cccctgtgag ggcacagcag ggttgcacat accagttccg gtcactgcaa agcttcttca
      241 ctttgaacat tctggtgatg tcctcagcag tcagagctcc aggcatgtct tgtttgttgg
      301 ctaatagaac aacaggcaca tttttaatgt gttcattctt caaaatgtgc tcaaactgtc
      361 tctgagactc ttccagtcgc tgtttgtctg tactgtccac aacatacacc agcccatcgg
      421 tgttctcaca gtaacagccc caaacagttc tcattttttc ctgtcctcca acatcccaga
      481 ctgtgagtga aaaattcctt tccaactcga tcatttccac attgaaacct attgtaggga
      541 tggtggtaat atccttagca agctttaatt tataaaggag agtagacttc ccagctgagt
      601 caagtcccaa aagaagaact tgggcttgtt tggtttgcgg atttttagaa cccagcgaac
      661 ccatggttct atctccttct aagcctgctt ttttgtacaa acttgtttga ttcgagtgtc
      721 gacggtatcg ataagcttga tatcgaattc ctgcagcccg ggggatccgc ccgggctaga
      781 gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt taatttcgag
      841 cttggcgtaa tcgctagcgg atctgacggt tcactaaacc agctctgct
//
