LOCUS       sequence-121320-         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 GTF2A1L
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..37)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(180..199)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(181..199)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        231..335
                     /gene="bla"
                     /label=AmpR promoter
     CDS             520..898
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(554..573)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ccttgcttgt gccgtggtga tggtggtgat ggtggtgacc agaggtgccc atggtttcac
       61 ttacgtgttt aatgtcttta cagactttat acattactgt catggttaaa taaaataaaa
      121 taaaataaaa taaaataaaa taaaataaaa taaaataaaa taaaataaac ttaagatgtc
      181 cctatagtga gtcgtattag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      241 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      301 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
      361 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
      421 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
      481 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
      541 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
      601 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
      661 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
      721 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
      781 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
      841 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
      901 gcaaactatt aactggcgaa ctacttactc tagcttcccc ggcaacaatt aatagactgg
      961 atgga
//
