LOCUS       sequence-121321-         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for cell-free expression with N-term 
            8xHis mCherry tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Gagoski D, Mureev S, Giles N, Johnston W, Dahmer-Heath M, Skalamera 
            D, Gonda TJ, Alexandrov K
  TITLE     Gateway-compatible vectors for high-throughput protein expression in
            pro- and eukaryotic cell-free systems.
  JOURNAL   J Biotechnol. 2015 Feb 10;195:1-7. doi: 
            10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19.
  PUBMED    25529348
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Biotechnol."; date: "2015-02-10"; volume: "195:1-7. doi"; pages: " 
            10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19"
COMMENT     Sequence Label: pCellFree_G05
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        330..432
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(499..518)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc actttgtaca
      181 agaaagcttg ctaagttggc agcatcaccc gacgcacttt gcgccgaata aatacctgtg
      241 acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac cgggaagccc
      301 tgggccaact tttggcgaaa atgagacgtt gatcggcacg caagaggttc caactttcac
      361 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
      421 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
      481 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
      541 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
      601 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
      661 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
      721 gagcaaactg aaacgttttc atcgctctgg agtgaaatac cacgacgatt tccggcagtt
      781 tctacacata tattcgcaag atgtggcgtg gttacggtga aaacctggcc tatttcccta
      841 aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt tttcaccagt
      901 tttgatttaa cgtggccaat atggacaact tc
//
