LOCUS       sequence-121347-        5776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NanoLuc expressed constiutively from PAM1 and RC500 RBS, pNBU2 
            backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 5776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2015.06"; journalName: "Cell Syst"; date: 
            "2015-07-29- 29"; volume: "1"; issue: "1"; pages: "62-71"
COMMENT     Sequence Label: pAT776
FEATURES             Location/Qualifiers
     source          1..5776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(3055..3123,3124..3915)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3273..3292)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            4046..4155
                     /note="incP origin of transfer"
     rep_origin      4326..4714
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 atccatcaaa attcgttttc tctatgttgg attccttgtt gctcatattg tgatgataat
       61 ttctacaaat atagtcattg gtaactatct atgaaactgt ttgatacttt tatagttgat
      121 taaacttgtt catggcattt gccttaatat catccgctat gtcaatgtag ggtttcatag
      181 ctttgtagtc gctgtgtccc gtccatttca tgaccacctg tgccgggatt ccgagagcca
      241 gcgcattgca gatgaatgtc cttcttcctg catgggtact gagcaaagcg tatttgggtg
      301 tgacttcatc aatacgttca tttcccttgt agtaggtttc ccgtacaggc tcgttgattt
      361 ctgccagttc gcccagctct ttcaggtaat cgttcatctt ctggttgctg atgacgggca
      421 gagccatgta attctcgaaa tggatgtcct tgtatttgtc cagtatggct ttgctgtatt
      481 tgttcagttc aatcgtcagg ctgtcggcag tcttgactgt ggttatttcg atgtggtcgg
      541 acttcacatc gcttcttttc agattgcgaa catccgaata ccgcaaactc gtaaagcagc
      601 agaacaggaa aacatcacgc acacgttcca ggtattgctt atccttgggt atctggtagt
      661 ctttcagctt gttcagttca tcccaagtca ggaagattac ttttttcgag gtggttttca
      721 gtttcggttt gaacgtatcg tatgcaatgt tctgatgatg tcctttcttg aagctccagc
      781 gcaggaacca tttgaggaat cccatttgct tgccgatggt gctgtttctc atatccttgg
      841 tgtcacgcag gaagttgacg tattcgttca atccaaactc gttgaaatag ttgaacgttg
      901 catcctcctt gaactctttg aggtggttcc tcactgctgc aaatttttca taggtggatg
      961 ccgtccagtt attctggtta ccgcactctt ttacaaactc atcgaacacc tcccaaaagc
     1021 tgacaggggc ttcttccggc tgttcttcgc tggtgtcttt cattctcatg ttgaaagctt
     1081 ccttcaactg ttgggtcgtt ggcatgacct cctgcacctc aaattccttg aaaatattct
     1141 ggatttcggc atagtatttc agcaagtccg tattgatttc ggctgcactt tgctttagct
     1201 tgttggtaca tccgctcttt acccgctgct tatctgcatc ccatttggct acgtcaatcc
     1261 ggtagcccgt tgtaaactcg atgcgttggc tggcaaagat gacacgcata cggatgggta
     1321 cgttctctac gattggcaca ccgttctttt tccggctctc caatgcaaaa atgatgttgc
     1381 gcttgatatt cataattggg tgcgtttgaa attctacacc caaatataca cccaattatt
     1441 gagatagcaa aagacattta gaaacattta cttttactct atattgtaat ttacacttga
     1501 ttatcagtcg tttgcagtct tatgatattc tgtgaaagta taagttcgag agcctgtctc
     1561 tccgcaaaaa acgctgaaaa tcagcagatt gcaaaacaaa caccctgttt tacacccaag
     1621 aatgtaaagt cggctgtttt tgttttattt aagataatac aaccactaca taataaaaga
     1681 gtagcgatat taaaagaatc cgatgagaaa agactaatat ttatctatcc attcagtttg
     1741 atttttcagg actttacatc gtcctgaaag tatttgttgg taccggtacc gaggacgcgt
     1801 aaacatttac agttgcatgt ggcctattgt ttttagccgt taaatatttt ataactatta
     1861 aatagcgata caaattgttc gaaactaata ttgtttatat catatattct cgcatgtttt
     1921 aaagctttat taaattgatt ttttgtaaac agtttttcgt actctttgtt aacccatttc
     1981 attacaaaag tttcatattt ttttctctct ttaaatgcca tttttgctgg ctttcttttt
     2041 aatacaatta atgtgctatc cactttaggt tttggatgga aataatacct aggaattttt
     2101 gctaatatag aaatatctac ctctgccatt aacagcaatg ctagtgatct gtttgtatct
     2161 aataacattt tagcaaaacc atattccact attaaataac ttattgtggc tgaactttca
     2221 aaaacaattt ttcgaattat atttgtgctt atgttgtaag gtatgctgcc aaatatttta
     2281 tatggattgt ggctaggaaa tgtaaatttc agtatatcat catttactat ttgatagtta
     2341 ggataattta agagcttatt acgagttacc tcacataatt tagaatcaat ttctatcgcc
     2401 gttacaaaat tacatctctt taccaatcca gcagtaaaat gacctttccc tgcacctatt
     2461 tcaaagatgt tatctttttc atctaaactt atgcaattca ttattttttc tatgtgatat
     2521 tttgaagtaa taaaattttg actatctttt atatttactt tgttcattat aacctctcct
     2581 taatttattg catctctttt cgaatattta tgttttttga gaaaagaacg tactcatggt
     2641 tcatcccgat atgcgtatcg gtctgtatat cagcaacttt ctatgtgttt caactacaat
     2701 agtcatctat tctcatcttt ctgagtccac cccctgcaaa gcccctcttt acgacataaa
     2761 aattcggtcg gaaaaggtat gcaaaagatg tttctctctt taagagaaac tcttcgggat
     2821 gcaaaaatat gaaaataact ccaattcacc aaattatata gcgacttttt tacaaaatgc
     2881 taaaatttgt tgatttccgt caagcaattg ttgagcaaaa atgtctttta cgataaaatg
     2941 atacctcaat atcaactgtt tagcaaaacg atatttctct taaagagaga aacacctttt
     3001 tgttcaccaa tccccgactt ttaatcccgc ggccatgatt gaaaaaggaa gagtatgagt
     3061 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     3121 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     3181 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     3241 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     3301 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     3361 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     3421 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     3481 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     3541 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     3601 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     3661 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     3721 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     3781 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     3841 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     3901 attaagcatt ggtaactgtc agaccaagtt tactcataac gcgtcaattc gagggggatc
     3961 aattccgtga taggtgggct gcccttcctg gttggcttgg tttcatcagc catccgcttg
     4021 ccctcatctg ttacgccggc ggtagccggc cagcctcgca gagcaggatt cccgttgagc
     4081 accgccaggt gcgaataagg gacagtgaag aaggaacacc cgctcgcggg tgggcctact
     4141 tcacctatcc tgcccggctg acgccgttgg atacaccaag gaaagtctac acgaaccctt
     4201 tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat cgctataatg
     4261 accccgaagc agggttatgc agcggaaaac ggaattgatc cggccacgat gcgtccggcg
     4321 tagaggatct gaagatcagc agttcaacct gttgatagta cgtactaagc tctcatgttt
     4381 cacgtactaa gctctcatgt ttaacgtact aagctctcat gtttaacgaa ctaaaccctc
     4441 atggctaacg tactaagctc tcatggctaa cgtactaagc tctcatgttt cacgtactaa
     4501 gctctcatgt ttgaacaata aaattaatat aaatcagcaa cttaaatagc ctctaaggtt
     4561 ttaagtttta taagaaaaaa aagaatatat aaggctttta aagcttttaa ggtttaacgg
     4621 ttgtggacaa caagccaggg atgtaacgca ctgagaagcc cttagagcct ctcaaagcaa
     4681 ttttgagtga cacaggaaca cttaacggct gacatgggaa ttcccctcca ccgcggtggc
     4741 ggccgctgat ctggaagaag caatgaaagc tgctgttaag tctccgaatc aggtattgtt
     4801 cctgacaggt gtattcccat ccggtaaacg cggatacttt gcagttgatc tgactcagga
     4861 ataaattata aattaaggta agaagattgt aggataagct aatgaaatag aaaaaggatg
     4921 ccgtcacaca acttgtcggc attctttttt gctttgcaac agcatagctc agcacagaag
     4981 ttgcctaaat atgtatgtta acaaattatt tgtcgtaact ttgcactcca ataggccttt
     5041 cggtccacac tctctatagg caaaatggtt tttactctgg aagattttgt tggcgattgg
     5101 cgtcagaccg cgggttataa tttggatcaa gtcctggaac agggtggcgt aagctctctg
     5161 ttccagaacc tgggtgtgag cgtgacgccg attcagcgca tcgttctgtc cggcgagaac
     5221 ggtctgaaaa ttgatattca tgtgatcatc ccgtacgaag gcctgagcgg tgaccaaatg
     5281 ggtcaaatcg agaaaatctt taaagtcgtc tacccagttg acgatcacca cttcaaggtt
     5341 atcttgcatt acggtacgct ggtgattgat ggtgtgaccc cgaatatgat tgactatttc
     5401 ggccgtccgt atgaaggcat tgccgttttt gacggtaaaa agatcaccgt caccggtacc
     5461 ctgtggaatg gcaataagat tattgacgag cgtctgatta acccggacgg cagcctgctg
     5521 ttccgcgtga ccatcaacgg tgtcacgggt tggcgtctgt gcgagcgcat cctggcataa
     5581 tgaactgcac ttgctttgat aattaatgat aaacaatcta aaagcactct aatcgttatc
     5641 ggagtgcttt tagattacta atcaaattgc ttctactaat tgcctatctt ccagtgatgg
     5701 aacagcattt gtgcattggc tgcaacaatc agccttactt gtgcctgttc tatttccgaa
     5761 ccgaccgctt gtatga
//
