LOCUS       sequence-121366-        5638 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NanoLuc expressed constiutively from PcfiA, pNBU2 backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2015.06"; journalName: "Cell Syst"; date: 
            "2015-07-29- 29"; volume: "1"; issue: "1"; pages: "62-71"
COMMENT     Sequence Label: pMM575
FEATURES             Location/Qualifiers
     source          1..5638
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(3954..4022,4023..4814)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4172..4191)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            4945..5054
                     /note="incP origin of transfer"
     rep_origin      5225..5613
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 ggagtgagct tctcggattt tatttgtatt tttgccatgc ctgatgaggt tttgtttgat
       61 tatttttttg caacactaag ttaagtgaat cctctgacat ggcaaaatcc tgagcaactt
      121 tttgttgctc aggtacttaa aaaaaatatt ttataatagt gttgcggaat taaggtaaaa
      181 gaataaaatg gtttttactc tggaagattt tgttggcgat tggcgtcaga ccgcgggtta
      241 taatttggat caagtcctgg aacagggtgg cgtaagctct ctgttccaga acctgggtgt
      301 gagcgtgacg ccgattcagc gcatcgttct gtccggcgag aacggtctga aaattgatat
      361 tcatgtgatc atcccgtacg aaggcctgag cggtgaccaa atgggtcaaa tcgagaaaat
      421 ctttaaagtc gtctacccag ttgacgatca ccacttcaag gttatcttgc attacggtac
      481 gctggtgatt gatggtgtga ccccgaatat gattgactat ttcggccgtc cgtatgaagg
      541 cattgccgtt tttgacggta aaaagatcac cgtcaccggt accctgtgga atggcaataa
      601 gattattgac gagcgtctga ttaacccgga cggcagcctg ctgttccgcg tgaccatcaa
      661 cggtgtcacg ggttggcgtc tgtgcgagcg catcctggca taatgaactg cacttgcttt
      721 gataattaat gataaacaat ctaaaagcac tctaatcgtt atcggagtgc ttttagatta
      781 ctaatcaaat tgcttctact aattgcctat cttccagtga tggaacagca tttgtgcatt
      841 ggctgcaaca atcagcctta cttgtgcctg ttctatttcc gaaccgaccg cttgtatgaa
      901 tccatcaaaa ttcgttttct ctatgttgga ttccttgttg ctcatattgt gatgataatt
      961 tctacaaata tagtcattgg taactatcta tgaaactgtt tgatactttt atagttgatt
     1021 aaacttgttc atggcatttg ccttaatatc atccgctatg tcaatgtagg gtttcatagc
     1081 tttgtagtcg ctgtgtcccg tccatttcat gaccacctgt gccgggattc cgagagccag
     1141 cgcattgcag atgaatgtcc ttcttcctgc atgggtactg agcaaagcgt atttgggtgt
     1201 gacttcatca atacgttcat ttcccttgta gtaggtttcc cgtacaggct cgttgatttc
     1261 tgccagttcg cccagctctt tcaggtaatc gttcatcttc tggttgctga tgacgggcag
     1321 agccatgtaa ttctcgaaat ggatgtcctt gtatttgtcc agtatggctt tgctgtattt
     1381 gttcagttca atcgtcaggc tgtcggcagt cttgactgtg gttatttcga tgtggtcgga
     1441 cttcacatcg cttcttttca gattgcgaac atccgaatac cgcaaactcg taaagcagca
     1501 gaacaggaaa acatcacgca cacgttccag gtattgctta tccttgggta tctggtagtc
     1561 tttcagcttg ttcagttcat cccaagtcag gaagattact tttttcgagg tggttttcag
     1621 tttcggtttg aacgtatcgt atgcaatgtt ctgatgatgt cctttcttga agctccagcg
     1681 caggaaccat ttgaggaatc ccatttgctt gccgatggtg ctgtttctca tatccttggt
     1741 gtcacgcagg aagttgacgt attcgttcaa tccaaactcg ttgaaatagt tgaacgttgc
     1801 atcctccttg aactctttga ggtggttcct cactgctgca aatttttcat aggtggatgc
     1861 cgtccagtta ttctggttac cgcactcttt tacaaactca tcgaacacct cccaaaagct
     1921 gacaggggct tcttccggct gttcttcgct ggtgtctttc attctcatgt tgaaagcttc
     1981 cttcaactgt tgggtcgttg gcatgacctc ctgcacctca aattccttga aaatattctg
     2041 gatttcggca tagtatttca gcaagtccgt attgatttcg gctgcacttt gctttagctt
     2101 gttggtacat ccgctcttta cccgctgctt atctgcatcc catttggcta cgtcaatccg
     2161 gtagcccgtt gtaaactcga tgcgttggct ggcaaagatg acacgcatac ggatgggtac
     2221 gttctctacg attggcacac cgttcttttt ccggctctcc aatgcaaaaa tgatgttgcg
     2281 cttgatattc ataattgggt gcgtttgaaa ttctacaccc aaatatacac ccaattattg
     2341 agatagcaaa agacatttag aaacatttac ttttactcta tattgtaatt tacacttgat
     2401 tatcagtcgt ttgcagtctt atgatattct gtgaaagtat aagttcgaga gcctgtctct
     2461 ccgcaaaaaa cgctgaaaat cagcagattg caaaacaaac accctgtttt acacccaaga
     2521 atgtaaagtc ggctgttttt gttttattta agataataca accactacat aataaaagag
     2581 tagcgatatt aaaagaatcc gatgagaaaa gactaatatt tatctatcca ttcagtttga
     2641 tttttcagga ctttacatcg tcctgaaagt atttgttggt accggtaccg aggacgcgta
     2701 aacatttaca gttgcatgtg gcctattgtt tttagccgtt aaatatttta taactattaa
     2761 atagcgatac aaattgttcg aaactaatat tgtttatatc atatattctc gcatgtttta
     2821 aagctttatt aaattgattt tttgtaaaca gtttttcgta ctctttgtta acccatttca
     2881 ttacaaaagt ttcatatttt tttctctctt taaatgccat ttttgctggc tttcttttta
     2941 atacaattaa tgtgctatcc actttaggtt ttggatggaa ataataccta ggaatttttg
     3001 ctaatataga aatatctacc tctgccatta acagcaatgc tagtgatctg tttgtatcta
     3061 ataacatttt agcaaaacca tattccacta ttaaataact tattgtggct gaactttcaa
     3121 aaacaatttt tcgaattata tttgtgctta tgttgtaagg tatgctgcca aatattttat
     3181 atggattgtg gctaggaaat gtaaatttca gtatatcatc atttactatt tgatagttag
     3241 gataatttaa gagcttatta cgagttacct cacataattt agaatcaatt tctatcgccg
     3301 ttacaaaatt acatctcttt accaatccag cagtaaaatg acctttccct gcacctattt
     3361 caaagatgtt atctttttca tctaaactta tgcaattcat tattttttct atgtgatatt
     3421 ttgaagtaat aaaattttga ctatctttta tatttacttt gttcattata acctctcctt
     3481 aatttattgc atctcttttc gaatatttat gttttttgag aaaagaacgt actcatggtt
     3541 catcccgata tgcgtatcgg tctgtatatc agcaactttc tatgtgtttc aactacaata
     3601 gtcatctatt ctcatctttc tgagtccacc ccctgcaaag cccctcttta cgacataaaa
     3661 attcggtcgg aaaaggtatg caaaagatgt ttctctcttt aagagaaact cttcgggatg
     3721 caaaaatatg aaaataactc caattcacca aattatatag cgactttttt acaaaatgct
     3781 aaaatttgtt gatttccgtc aagcaattgt tgagcaaaaa tgtcttttac gataaaatga
     3841 tacctcaata tcaactgttt agcaaaacga tatttctctt aaagagagaa acaccttttt
     3901 gttcaccaat ccccgacttt taatcccgcg gccatgattg aaaaaggaag agtatgagta
     3961 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4021 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4081 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     4141 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     4201 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     4261 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     4321 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     4381 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     4441 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     4501 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     4561 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     4621 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     4681 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     4741 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     4801 ttaagcattg gtaactgtca gaccaagttt actcataacg cgtcaattcg agggggatca
     4861 attccgtgat aggtgggctg cccttcctgg ttggcttggt ttcatcagcc atccgcttgc
     4921 cctcatctgt tacgccggcg gtagccggcc agcctcgcag agcaggattc ccgttgagca
     4981 ccgccaggtg cgaataaggg acagtgaaga aggaacaccc gctcgcgggt gggcctactt
     5041 cacctatcct gcccggctga cgccgttgga tacaccaagg aaagtctaca cgaacccttt
     5101 ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac cgaaaaaatc gctataatga
     5161 ccccgaagca gggttatgca gcggaaaacg gaattgatcc ggccacgatg cgtccggcgt
     5221 agaggatctg aagatcagca gttcaacctg ttgatagtac gtactaagct ctcatgtttc
     5281 acgtactaag ctctcatgtt taacgtacta agctctcatg tttaacgaac taaaccctca
     5341 tggctaacgt actaagctct catggctaac gtactaagct ctcatgtttc acgtactaag
     5401 ctctcatgtt tgaacaataa aattaatata aatcagcaac ttaaatagcc tctaaggttt
     5461 taagttttat aagaaaaaaa agaatatata aggcttttaa agcttttaag gtttaacggt
     5521 tgtggacaac aagccaggga tgtaacgcac tgagaagccc ttagagcctc tcaaagcaat
     5581 tttgagtgac acaggaacac ttaacggctg acatgggaat tcccctccac cgcggtgg
//
