LOCUS       sequence-121367-        5530 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NanoLuc expressed constiutively from P1, pNBU2 backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2015.06"; journalName: "Cell Syst"; date: 
            "2015-07-29- 29"; volume: "1"; issue: "1"; pages: "62-71"
COMMENT     Sequence Label: pMM579
FEATURES             Location/Qualifiers
     source          1..5530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(3811..3879,3880..4671)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4029..4048)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            4802..4911
                     /note="incP origin of transfer"
     rep_origin      5082..5470
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 ctttgcagtc cgaaataaag acatataaaa gaaaagacac catgatggtt tttactctgg
       61 aagattttgt tggcgattgg cgtcagaccg cgggttataa tttggatcaa gtcctggaac
      121 agggtggcgt aagctctctg ttccagaacc tgggtgtgag cgtgacgccg attcagcgca
      181 tcgttctgtc cggcgagaac ggtctgaaaa ttgatattca tgtgatcatc ccgtacgaag
      241 gcctgagcgg tgaccaaatg ggtcaaatcg agaaaatctt taaagtcgtc tacccagttg
      301 acgatcacca cttcaaggtt atcttgcatt acggtacgct ggtgattgat ggtgtgaccc
      361 cgaatatgat tgactatttc ggccgtccgt atgaaggcat tgccgttttt gacggtaaaa
      421 agatcaccgt caccggtacc ctgtggaatg gcaataagat tattgacgag cgtctgatta
      481 acccggacgg cagcctgctg ttccgcgtga ccatcaacgg tgtcacgggt tggcgtctgt
      541 gcgagcgcat cctggcataa tgaactgcac ttgctttgat aattaatgat aaacaatcta
      601 aaagcactct aatcgttatc ggagtgcttt tagattacta atcaaattgc ttctactaat
      661 tgcctatctt ccagtgatgg aacagcattt gtgcattggc tgcaacaatc agccttactt
      721 gtgcctgttc tatttccgaa ccgaccgctt gtatgaatcc atcaaaattc gttttctcta
      781 tgttggattc cttgttgctc atattgtgat gataatttct acaaatatag tcattggtaa
      841 ctatctatga aactgtttga tacttttata gttgattaaa cttgttcatg gcatttgcct
      901 taatatcatc cgctatgtca atgtagggtt tcatagcttt gtagtcgctg tgtcccgtcc
      961 atttcatgac cacctgtgcc gggattccga gagccagcgc attgcagatg aatgtccttc
     1021 ttcctgcatg ggtactgagc aaagcgtatt tgggtgtgac ttcatcaata cgttcatttc
     1081 ccttgtagta ggtttcccgt acaggctcgt tgatttctgc cagttcgccc agctctttca
     1141 ggtaatcgtt catcttctgg ttgctgatga cgggcagagc catgtaattc tcgaaatgga
     1201 tgtccttgta tttgtccagt atggctttgc tgtatttgtt cagttcaatc gtcaggctgt
     1261 cggcagtctt gactgtggtt atttcgatgt ggtcggactt cacatcgctt cttttcagat
     1321 tgcgaacatc cgaataccgc aaactcgtaa agcagcagaa caggaaaaca tcacgcacac
     1381 gttccaggta ttgcttatcc ttgggtatct ggtagtcttt cagcttgttc agttcatccc
     1441 aagtcaggaa gattactttt ttcgaggtgg ttttcagttt cggtttgaac gtatcgtatg
     1501 caatgttctg atgatgtcct ttcttgaagc tccagcgcag gaaccatttg aggaatccca
     1561 tttgcttgcc gatggtgctg tttctcatat ccttggtgtc acgcaggaag ttgacgtatt
     1621 cgttcaatcc aaactcgttg aaatagttga acgttgcatc ctccttgaac tctttgaggt
     1681 ggttcctcac tgctgcaaat ttttcatagg tggatgccgt ccagttattc tggttaccgc
     1741 actcttttac aaactcatcg aacacctccc aaaagctgac aggggcttct tccggctgtt
     1801 cttcgctggt gtctttcatt ctcatgttga aagcttcctt caactgttgg gtcgttggca
     1861 tgacctcctg cacctcaaat tccttgaaaa tattctggat ttcggcatag tatttcagca
     1921 agtccgtatt gatttcggct gcactttgct ttagcttgtt ggtacatccg ctctttaccc
     1981 gctgcttatc tgcatcccat ttggctacgt caatccggta gcccgttgta aactcgatgc
     2041 gttggctggc aaagatgaca cgcatacgga tgggtacgtt ctctacgatt ggcacaccgt
     2101 tctttttccg gctctccaat gcaaaaatga tgttgcgctt gatattcata attgggtgcg
     2161 tttgaaattc tacacccaaa tatacaccca attattgaga tagcaaaaga catttagaaa
     2221 catttacttt tactctatat tgtaatttac acttgattat cagtcgtttg cagtcttatg
     2281 atattctgtg aaagtataag ttcgagagcc tgtctctccg caaaaaacgc tgaaaatcag
     2341 cagattgcaa aacaaacacc ctgttttaca cccaagaatg taaagtcggc tgtttttgtt
     2401 ttatttaaga taatacaacc actacataat aaaagagtag cgatattaaa agaatccgat
     2461 gagaaaagac taatatttat ctatccattc agtttgattt ttcaggactt tacatcgtcc
     2521 tgaaagtatt tgttggtacc ggtaccgagg acgcgtaaac atttacagtt gcatgtggcc
     2581 tattgttttt agccgttaaa tattttataa ctattaaata gcgatacaaa ttgttcgaaa
     2641 ctaatattgt ttatatcata tattctcgca tgttttaaag ctttattaaa ttgatttttt
     2701 gtaaacagtt tttcgtactc tttgttaacc catttcatta caaaagtttc atattttttt
     2761 ctctctttaa atgccatttt tgctggcttt ctttttaata caattaatgt gctatccact
     2821 ttaggttttg gatggaaata atacctagga atttttgcta atatagaaat atctacctct
     2881 gccattaaca gcaatgctag tgatctgttt gtatctaata acattttagc aaaaccatat
     2941 tccactatta aataacttat tgtggctgaa ctttcaaaaa caatttttcg aattatattt
     3001 gtgcttatgt tgtaaggtat gctgccaaat attttatatg gattgtggct aggaaatgta
     3061 aatttcagta tatcatcatt tactatttga tagttaggat aatttaagag cttattacga
     3121 gttacctcac ataatttaga atcaatttct atcgccgtta caaaattaca tctctttacc
     3181 aatccagcag taaaatgacc tttccctgca cctatttcaa agatgttatc tttttcatct
     3241 aaacttatgc aattcattat tttttctatg tgatattttg aagtaataaa attttgacta
     3301 tcttttatat ttactttgtt cattataacc tctccttaat ttattgcatc tcttttcgaa
     3361 tatttatgtt ttttgagaaa agaacgtact catggttcat cccgatatgc gtatcggtct
     3421 gtatatcagc aactttctat gtgtttcaac tacaatagtc atctattctc atctttctga
     3481 gtccaccccc tgcaaagccc ctctttacga cataaaaatt cggtcggaaa aggtatgcaa
     3541 aagatgtttc tctctttaag agaaactctt cgggatgcaa aaatatgaaa ataactccaa
     3601 ttcaccaaat tatatagcga cttttttaca aaatgctaaa atttgttgat ttccgtcaag
     3661 caattgttga gcaaaaatgt cttttacgat aaaatgatac ctcaatatca actgtttagc
     3721 aaaacgatat ttctcttaaa gagagaaaca cctttttgtt caccaatccc cgacttttaa
     3781 tcccgcggcc atgattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     3841 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     3901 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     3961 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     4021 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     4081 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     4141 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     4201 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     4261 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     4321 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     4381 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     4441 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     4501 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     4561 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     4621 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     4681 caagtttact cataacgcgt caattcgagg gggatcaatt ccgtgatagg tgggctgccc
     4741 ttcctggttg gcttggtttc atcagccatc cgcttgccct catctgttac gccggcggta
     4801 gccggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga ataagggaca
     4861 gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc cggctgacgc
     4921 cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt atatcgtgcg
     4981 aaaaaggatg gatataccga aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg
     5041 gaaaacggaa ttgatccggc cacgatgcgt ccggcgtaga ggatctgaag atcagcagtt
     5101 caacctgttg atagtacgta ctaagctctc atgtttcacg tactaagctc tcatgtttaa
     5161 cgtactaagc tctcatgttt aacgaactaa accctcatgg ctaacgtact aagctctcat
     5221 ggctaacgta ctaagctctc atgtttcacg tactaagctc tcatgtttga acaataaaat
     5281 taatataaat cagcaactta aatagcctct aaggttttaa gttttataag aaaaaaaaga
     5341 atatataagg cttttaaagc ttttaaggtt taacggttgt ggacaacaag ccagggatgt
     5401 aacgcactga gaagccctta gagcctctca aagcaatttt gagtgacaca ggaacactta
     5461 acggctgaca tgggaattcc cctccaccgc ggtgggataa agtttggaag ataaagctaa
     5521 aagttcttat
//
