LOCUS       sequence-121373-        5647 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rhamnose-inducible NanoLuc expression, pNBU2 backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 5647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2015.06"; journalName: "Cell Syst"; date: 
            "2015-07-29- 29"; volume: "1"; issue: "1"; pages: "62-71"
COMMENT     Sequence Label: pMM656
FEATURES             Location/Qualifiers
     source          1..5647
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(3743..3811,3812..4603)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3961..3980)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            4734..4843
                     /note="incP origin of transfer"
     rep_origin      5014..5402
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 gttggcgatt ggcgtcagac cgcgggttat aatttggatc aagtcctgga acagggtggc
       61 gtaagctctc tgttccagaa cctgggtgtg agcgtgacgc cgattcagcg catcgttctg
      121 tccggcgaga acggtctgaa aattgatatt catgtgatca tcccgtacga aggcctgagc
      181 ggtgaccaaa tgggtcaaat cgagaaaatc tttaaagtcg tctacccagt tgacgatcac
      241 cacttcaagg ttatcttgca ttacggtacg ctggtgattg atggtgtgac cccgaatatg
      301 attgactatt tcggccgtcc gtatgaaggc attgccgttt ttgacggtaa aaagatcacc
      361 gtcaccggta ccctgtggaa tggcaataag attattgacg agcgtctgat taacccggac
      421 ggcagcctgc tgttccgcgt gaccatcaac ggtgtcacgg gttggcgtct gtgcgagcgc
      481 atcctggcat aatgaactgc acttgctttg ataattaatg ataaacaatc taaaagcact
      541 ctaatcgtta tcggagtgct tttagattac taatcaaatt gcttctacta attgcctatc
      601 ttccagtgat ggaacagcat ttgtgcattg gctgcaacaa tcagccttac ttgtgcctgt
      661 tctatttccg aaccgaccgc ttgtatgaat ccatcaaaat tcgttttctc tatgttggat
      721 tccttgttgc tcatattgtg atgataattt ctacaaatat agtcattggt aactatctat
      781 gaaactgttt gatactttta tagttgatta aacttgttca tggcatttgc cttaatatca
      841 tccgctatgt caatgtaggg tttcatagct ttgtagtcgc tgtgtcccgt ccatttcatg
      901 accacctgtg ccgggattcc gagagccagc gcattgcaga tgaatgtcct tcttcctgca
      961 tgggtactga gcaaagcgta tttgggtgtg acttcatcaa tacgttcatt tcccttgtag
     1021 taggtttccc gtacaggctc gttgatttct gccagttcgc ccagctcttt caggtaatcg
     1081 ttcatcttct ggttgctgat gacgggcaga gccatgtaat tctcgaaatg gatgtccttg
     1141 tatttgtcca gtatggcttt gctgtatttg ttcagttcaa tcgtcaggct gtcggcagtc
     1201 ttgactgtgg ttatttcgat gtggtcggac ttcacatcgc ttcttttcag attgcgaaca
     1261 tccgaatacc gcaaactcgt aaagcagcag aacaggaaaa catcacgcac acgttccagg
     1321 tattgcttat ccttgggtat ctggtagtct ttcagcttgt tcagttcatc ccaagtcagg
     1381 aagattactt ttttcgaggt ggttttcagt ttcggtttga acgtatcgta tgcaatgttc
     1441 tgatgatgtc ctttcttgaa gctccagcgc aggaaccatt tgaggaatcc catttgcttg
     1501 ccgatggtgc tgtttctcat atccttggtg tcacgcagga agttgacgta ttcgttcaat
     1561 ccaaactcgt tgaaatagtt gaacgttgca tcctccttga actctttgag gtggttcctc
     1621 actgctgcaa atttttcata ggtggatgcc gtccagttat tctggttacc gcactctttt
     1681 acaaactcat cgaacacctc ccaaaagctg acaggggctt cttccggctg ttcttcgctg
     1741 gtgtctttca ttctcatgtt gaaagcttcc ttcaactgtt gggtcgttgg catgacctcc
     1801 tgcacctcaa attccttgaa aatattctgg atttcggcat agtatttcag caagtccgta
     1861 ttgatttcgg ctgcactttg ctttagcttg ttggtacatc cgctctttac ccgctgctta
     1921 tctgcatccc atttggctac gtcaatccgg tagcccgttg taaactcgat gcgttggctg
     1981 gcaaagatga cacgcatacg gatgggtacg ttctctacga ttggcacacc gttctttttc
     2041 cggctctcca atgcaaaaat gatgttgcgc ttgatattca taattgggtg cgtttgaaat
     2101 tctacaccca aatatacacc caattattga gatagcaaaa gacatttaga aacatttact
     2161 tttactctat attgtaattt acacttgatt atcagtcgtt tgcagtctta tgatattctg
     2221 tgaaagtata agttcgagag cctgtctctc cgcaaaaaac gctgaaaatc agcagattgc
     2281 aaaacaaaca ccctgtttta cacccaagaa tgtaaagtcg gctgtttttg ttttatttaa
     2341 gataatacaa ccactacata ataaaagagt agcgatatta aaagaatccg atgagaaaag
     2401 actaatattt atctatccat tcagtttgat ttttcaggac tttacatcgt cctgaaagta
     2461 tttgttggta ccggtaccga ggacgcgtaa acatttacag ttgcatgtgg cctattgttt
     2521 ttagccgtta aatattttat aactattaaa tagcgataca aattgttcga aactaatatt
     2581 gtttatatca tatattctcg catgttttaa agctttatta aattgatttt ttgtaaacag
     2641 tttttcgtac tctttgttaa cccatttcat tacaaaagtt tcatattttt ttctctcttt
     2701 aaatgccatt tttgctggct ttctttttaa tacaattaat gtgctatcca ctttaggttt
     2761 tggatggaaa taatacctag gaatttttgc taatatagaa atatctacct ctgccattaa
     2821 cagcaatgct agtgatctgt ttgtatctaa taacatttta gcaaaaccat attccactat
     2881 taaataactt attgtggctg aactttcaaa aacaattttt cgaattatat ttgtgcttat
     2941 gttgtaaggt atgctgccaa atattttata tggattgtgg ctaggaaatg taaatttcag
     3001 tatatcatca tttactattt gatagttagg ataatttaag agcttattac gagttacctc
     3061 acataattta gaatcaattt ctatcgccgt tacaaaatta catctcttta ccaatccagc
     3121 agtaaaatga cctttccctg cacctatttc aaagatgtta tctttttcat ctaaacttat
     3181 gcaattcatt attttttcta tgtgatattt tgaagtaata aaattttgac tatcttttat
     3241 atttactttg ttcattataa cctctcctta atttattgca tctcttttcg aatatttatg
     3301 ttttttgaga aaagaacgta ctcatggttc atcccgatat gcgtatcggt ctgtatatca
     3361 gcaactttct atgtgtttca actacaatag tcatctattc tcatctttct gagtccaccc
     3421 cctgcaaagc ccctctttac gacataaaaa ttcggtcgga aaaggtatgc aaaagatgtt
     3481 tctctcttta agagaaactc ttcgggatgc aaaaatatga aaataactcc aattcaccaa
     3541 attatatagc gactttttta caaaatgcta aaatttgttg atttccgtca agcaattgtt
     3601 gagcaaaaat gtcttttacg ataaaatgat acctcaatat caactgttta gcaaaacgat
     3661 atttctctta aagagagaaa cacctttttg ttcaccaatc cccgactttt aatcccgcgg
     3721 ccatgattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     3781 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     3841 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     3901 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     3961 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     4021 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     4081 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     4141 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     4201 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     4261 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     4321 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     4381 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     4441 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     4501 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     4561 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     4621 ctcataacgc gtcaattcga gggggatcaa ttccgtgata ggtgggctgc ccttcctggt
     4681 tggcttggtt tcatcagcca tccgcttgcc ctcatctgtt acgccggcgg tagccggcca
     4741 gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc gaataaggga cagtgaagaa
     4801 ggaacacccg ctcgcgggtg ggcctacttc acctatcctg cccggctgac gccgttggat
     4861 acaccaagga aagtctacac gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga
     4921 tggatatacc gaaaaaatcg ctataatgac cccgaagcag ggttatgcag cggaaaacgg
     4981 aattgatccg gccacgatgc gtccggcgta gaggatctga agatcagcag ttcaacctgt
     5041 tgatagtacg tactaagctc tcatgtttca cgtactaagc tctcatgttt aacgtactaa
     5101 gctctcatgt ttaacgaact aaaccctcat ggctaacgta ctaagctctc atggctaacg
     5161 tactaagctc tcatgtttca cgtactaagc tctcatgttt gaacaataaa attaatataa
     5221 atcagcaact taaatagcct ctaaggtttt aagttttata agaaaaaaaa gaatatataa
     5281 ggcttttaaa gcttttaagg tttaacggtt gtggacaaca agccagggat gtaacgcact
     5341 gagaagccct tagagcctct caaagcaatt ttgagtgaca caggaacact taacggctga
     5401 catgggaatt cccctccacc gcggtggtat ttcggagaaa acatgcataa atcatgcttt
     5461 ttttgcataa aaagtaaaat ttatactgat gtaaggtttg gctatgcaga tttgtgtcaa
     5521 aatgcacatc ctttctatca aaatgcgtaa ggaaaaggag gaaggaaccg cctatctttg
     5581 caatgtaggt aaatggatac cttaaatata tagacaaaat accatggttt ttactctgga
     5641 agatttt
//
