LOCUS       sequence-121374-        5658 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Chondroitin sulfate-inducible NanoLuc expression, pNBU2 backbone, 
            AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2015.06"; journalName: "Cell Syst"; date: 
            "2015-07-29- 29"; volume: "1"; issue: "1"; pages: "62-71"
COMMENT     Sequence Label: pMM659
FEATURES             Location/Qualifiers
     source          1..5658
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(3738..3806,3807..4598)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3956..3975)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            4729..4838
                     /note="incP origin of transfer"
     rep_origin      5009..5397
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 cgattggcgt cagaccgcgg gttataattt ggatcaagtc ctggaacagg gtggcgtaag
       61 ctctctgttc cagaacctgg gtgtgagcgt gacgccgatt cagcgcatcg ttctgtccgg
      121 cgagaacggt ctgaaaattg atattcatgt gatcatcccg tacgaaggcc tgagcggtga
      181 ccaaatgggt caaatcgaga aaatctttaa agtcgtctac ccagttgacg atcaccactt
      241 caaggttatc ttgcattacg gtacgctggt gattgatggt gtgaccccga atatgattga
      301 ctatttcggc cgtccgtatg aaggcattgc cgtttttgac ggtaaaaaga tcaccgtcac
      361 cggtaccctg tggaatggca ataagattat tgacgagcgt ctgattaacc cggacggcag
      421 cctgctgttc cgcgtgacca tcaacggtgt cacgggttgg cgtctgtgcg agcgcatcct
      481 ggcataatga actgcacttg ctttgataat taatgataaa caatctaaaa gcactctaat
      541 cgttatcgga gtgcttttag attactaatc aaattgcttc tactaattgc ctatcttcca
      601 gtgatggaac agcatttgtg cattggctgc aacaatcagc cttacttgtg cctgttctat
      661 ttccgaaccg accgcttgta tgaatccatc aaaattcgtt ttctctatgt tggattcctt
      721 gttgctcata ttgtgatgat aatttctaca aatatagtca ttggtaacta tctatgaaac
      781 tgtttgatac ttttatagtt gattaaactt gttcatggca tttgccttaa tatcatccgc
      841 tatgtcaatg tagggtttca tagctttgta gtcgctgtgt cccgtccatt tcatgaccac
      901 ctgtgccggg attccgagag ccagcgcatt gcagatgaat gtccttcttc ctgcatgggt
      961 actgagcaaa gcgtatttgg gtgtgacttc atcaatacgt tcatttccct tgtagtaggt
     1021 ttcccgtaca ggctcgttga tttctgccag ttcgcccagc tctttcaggt aatcgttcat
     1081 cttctggttg ctgatgacgg gcagagccat gtaattctcg aaatggatgt ccttgtattt
     1141 gtccagtatg gctttgctgt atttgttcag ttcaatcgtc aggctgtcgg cagtcttgac
     1201 tgtggttatt tcgatgtggt cggacttcac atcgcttctt ttcagattgc gaacatccga
     1261 ataccgcaaa ctcgtaaagc agcagaacag gaaaacatca cgcacacgtt ccaggtattg
     1321 cttatccttg ggtatctggt agtctttcag cttgttcagt tcatcccaag tcaggaagat
     1381 tacttttttc gaggtggttt tcagtttcgg tttgaacgta tcgtatgcaa tgttctgatg
     1441 atgtcctttc ttgaagctcc agcgcaggaa ccatttgagg aatcccattt gcttgccgat
     1501 ggtgctgttt ctcatatcct tggtgtcacg caggaagttg acgtattcgt tcaatccaaa
     1561 ctcgttgaaa tagttgaacg ttgcatcctc cttgaactct ttgaggtggt tcctcactgc
     1621 tgcaaatttt tcataggtgg atgccgtcca gttattctgg ttaccgcact cttttacaaa
     1681 ctcatcgaac acctcccaaa agctgacagg ggcttcttcc ggctgttctt cgctggtgtc
     1741 tttcattctc atgttgaaag cttccttcaa ctgttgggtc gttggcatga cctcctgcac
     1801 ctcaaattcc ttgaaaatat tctggatttc ggcatagtat ttcagcaagt ccgtattgat
     1861 ttcggctgca ctttgcttta gcttgttggt acatccgctc tttacccgct gcttatctgc
     1921 atcccatttg gctacgtcaa tccggtagcc cgttgtaaac tcgatgcgtt ggctggcaaa
     1981 gatgacacgc atacggatgg gtacgttctc tacgattggc acaccgttct ttttccggct
     2041 ctccaatgca aaaatgatgt tgcgcttgat attcataatt gggtgcgttt gaaattctac
     2101 acccaaatat acacccaatt attgagatag caaaagacat ttagaaacat ttacttttac
     2161 tctatattgt aatttacact tgattatcag tcgtttgcag tcttatgata ttctgtgaaa
     2221 gtataagttc gagagcctgt ctctccgcaa aaaacgctga aaatcagcag attgcaaaac
     2281 aaacaccctg ttttacaccc aagaatgtaa agtcggctgt ttttgtttta tttaagataa
     2341 tacaaccact acataataaa agagtagcga tattaaaaga atccgatgag aaaagactaa
     2401 tatttatcta tccattcagt ttgatttttc aggactttac atcgtcctga aagtatttgt
     2461 tggtaccggt accgaggacg cgtaaacatt tacagttgca tgtggcctat tgtttttagc
     2521 cgttaaatat tttataacta ttaaatagcg atacaaattg ttcgaaacta atattgttta
     2581 tatcatatat tctcgcatgt tttaaagctt tattaaattg attttttgta aacagttttt
     2641 cgtactcttt gttaacccat ttcattacaa aagtttcata tttttttctc tctttaaatg
     2701 ccatttttgc tggctttctt tttaatacaa ttaatgtgct atccacttta ggttttggat
     2761 ggaaataata cctaggaatt tttgctaata tagaaatatc tacctctgcc attaacagca
     2821 atgctagtga tctgtttgta tctaataaca ttttagcaaa accatattcc actattaaat
     2881 aacttattgt ggctgaactt tcaaaaacaa tttttcgaat tatatttgtg cttatgttgt
     2941 aaggtatgct gccaaatatt ttatatggat tgtggctagg aaatgtaaat ttcagtatat
     3001 catcatttac tatttgatag ttaggataat ttaagagctt attacgagtt acctcacata
     3061 atttagaatc aatttctatc gccgttacaa aattacatct ctttaccaat ccagcagtaa
     3121 aatgaccttt ccctgcacct atttcaaaga tgttatcttt ttcatctaaa cttatgcaat
     3181 tcattatttt ttctatgtga tattttgaag taataaaatt ttgactatct tttatattta
     3241 ctttgttcat tataacctct ccttaattta ttgcatctct tttcgaatat ttatgttttt
     3301 tgagaaaaga acgtactcat ggttcatccc gatatgcgta tcggtctgta tatcagcaac
     3361 tttctatgtg tttcaactac aatagtcatc tattctcatc tttctgagtc caccccctgc
     3421 aaagcccctc tttacgacat aaaaattcgg tcggaaaagg tatgcaaaag atgtttctct
     3481 ctttaagaga aactcttcgg gatgcaaaaa tatgaaaata actccaattc accaaattat
     3541 atagcgactt ttttacaaaa tgctaaaatt tgttgatttc cgtcaagcaa ttgttgagca
     3601 aaaatgtctt ttacgataaa atgatacctc aatatcaact gtttagcaaa acgatatttc
     3661 tcttaaagag agaaacacct ttttgttcac caatccccga cttttaatcc cgcggccatg
     3721 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     3781 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     3841 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     3901 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     3961 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     4021 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     4081 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     4141 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     4201 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     4261 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     4321 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     4381 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     4441 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     4501 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     4561 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     4621 aacgcgtcaa ttcgaggggg atcaattccg tgataggtgg gctgcccttc ctggttggct
     4681 tggtttcatc agccatccgc ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc
     4741 gcagagcagg attcccgttg agcaccgcca ggtgcgaata agggacagtg aagaaggaac
     4801 acccgctcgc gggtgggcct acttcaccta tcctgcccgg ctgacgccgt tggatacacc
     4861 aaggaaagtc tacacgaacc ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat
     4921 ataccgaaaa aatcgctata atgaccccga agcagggtta tgcagcggaa aacggaattg
     4981 atccggccac gatgcgtccg gcgtagagga tctgaagatc agcagttcaa cctgttgata
     5041 gtacgtacta agctctcatg tttcacgtac taagctctca tgtttaacgt actaagctct
     5101 catgtttaac gaactaaacc ctcatggcta acgtactaag ctctcatggc taacgtacta
     5161 agctctcatg tttcacgtac taagctctca tgtttgaaca ataaaattaa tataaatcag
     5221 caacttaaat agcctctaag gttttaagtt ttataagaaa aaaaagaata tataaggctt
     5281 ttaaagcttt taaggtttaa cggttgtgga caacaagcca gggatgtaac gcactgagaa
     5341 gcccttagag cctctcaaag caattttgag tgacacagga acacttaacg gctgacatgg
     5401 gaattcccct ccaccgcggt ggcggtcatt atttataaga gatagcacat aatttgaact
     5461 attttgtacg atttgaaccc ctctttccaa caaaagaggg gtttctttgc attcgggaga
     5521 agaacaagtg atctctctct gtaaataccg gctaatgata aaccgattta ccatcggacc
     5581 taaaacgata tattctatga taaagcaatc ttttactctg tcagtgacaa tggtttttac
     5641 tctggaagat tttgttgg
//
