LOCUS       sequence-121376-        4172 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pExchange(tdk) - B. thetaiotaomicron suicide vector expressing 
            thymidine kinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2015.06"; journalName: "Cell Syst"; date: 
            "2015-07-29- 29"; volume: "1"; issue: "1"; pages: "62-71"
COMMENT     Sequence Label: pMM668
FEATURES             Location/Qualifiers
     source          1..4172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(30..821,822..890))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     653..672
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(3354..3370)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3354..3370)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     rep_origin      3403..3791
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            3962..4071
                     /note="incP origin of transfer"
ORIGIN
        1 acgcgttatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
       61 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
      121 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
      181 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
      241 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
      301 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
      361 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
      421 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
      481 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
      541 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
      601 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
      661 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
      721 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
      781 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
      841 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
      901 tttcaatcat ggccgcggga ttaaaagtcg gggattggtg aacaaaaagg tgtttctctc
      961 tttaagagaa atatcgtttt gctaaacagt tgatattgag gtatcatttt atcgtaaaag
     1021 acatttttgc tcaacaattg cttgacggaa atcaacaaat tttagcattt tgtaaaaaag
     1081 tcgctatata atttggtgaa ttggagttat tttcatattt ttgcatcccg aagagtttct
     1141 cttaaagaga gaaacatctt ttgcatacct tttccgaccg aatttttatg tcgtaaagag
     1201 gggctttgca gggggtggac tcagaaagat gagaatagat gactattgta gttgaaacac
     1261 atagaaagtt gctgatatac agaccgatac gcatatcggg atgaaccatg agtacgttct
     1321 tttctcaaaa aacataaata ttcgaaaaga gatgcaataa attaaggaga ggttataatg
     1381 aacaaagtaa atataaaaga tagtcaaaat tttattactt caaaatatca catagaaaaa
     1441 ataatgaatt gcataagttt agatgaaaaa gataacatct ttgaaatagg tgcagggaaa
     1501 ggtcatttta ctgctggatt ggtaaagaga tgtaattttg taacggcgat agaaattgat
     1561 tctaaattat gtgaggtaac tcgtaataag ctcttaaatt atcctaacta tcaaatagta
     1621 aatgatgata tactgaaatt tacatttcct agccacaatc catataaaat atttggcagc
     1681 ataccttaca acataagcac aaatataatt cgaaaaattg tttttgaaag ttcagccaca
     1741 ataagttatt taatagtgga atatggtttt gctaaaatgt tattagatac aaacagatca
     1801 ctagcattgc tgttaatggc agaggtagat atttctatat tagcaaaaat tcctaggtat
     1861 tatttccatc caaaacctaa agtggatagc acattaattg tattaaaaag aaagccagca
     1921 aaaatggcat ttaaagagag aaaaaaatat gaaacttttg taatgaaatg ggttaacaaa
     1981 gagtacgaaa aactgtttac aaaaaatcaa tttaataaag ctttaaaaca tgcgagaata
     2041 tatgatataa acaatattag tttcgaacaa tttgtatcgc tatttaatag ttataaaata
     2101 tttaacggct aaaaacaata ggccacatgc aactgtaaat gtttacgcgt cctcggtacc
     2161 gcttcttcca caacagtctg cggttcctgt actatcacag gttcatcttc tcccggtgct
     2221 gtcgcttcat tctcttccac gaccttctct tctatcaccg gacattcaac ttcctctatt
     2281 acaggggctt gttcttcaac aatgggggct tcactttccg cttccgctac aggagaaggc
     2341 gaggcttcca ccggcacagc acttatctct tccgacaact gttccaaacg ctcctgcata
     2401 cgtaggatgc tccgcttcaa cagttcagac aaagtctgag tcggctcttt agaaaacgta
     2461 ttcatgagta gcttcagctc atgaacatcc agctcaatat ctgttaatag cttctgtttc
     2521 atttccatcc attgttatta tggtgcgaat gtaggaataa tttatgcaat aacttaaaat
     2581 aatggataat ttaacagttc ttgtacatcg aattgtttac ctttgtccac tcatttattt
     2641 aatcaagaaa gcacaaaaat cacatggtat tattttcaga agaccacata caagagacca
     2701 gaagaagagg tagaatagag gtaatctgcg gttcaatgtt ctccggaaag acggaggaat
     2761 tgattcgtag gatgaagaga gccaagttcg cacgccagcg tgtagagata ttcaaaccgg
     2821 ccatcgacac ccgttattcg gaagaagacg tggtatcaca cgacagtcat tccatcgcgt
     2881 ccacccctat cgattcatcg gctagtatcc tgttgttcac ttctgaaata gatgtagtcg
     2941 gcatcgacga agcacagttc tttgatgacg gactgattga cgtatgcagg cagcttgcca
     3001 ataacggcat ccgtgtcatc atcgccggac tggatatgga ctttaaaggg aatcccttcg
     3061 gaccgatgcc tcaattatgt gccattgccg atgaagtttc caaagttcat gcgatctgcg
     3121 tgaagtgcgg tgatctggca tctttctctc atcgtaccgt caagaatgac aagcaggtac
     3181 ttctaggaga gacagccgaa tacgagccgc tttgcaggga gtgctacctc cgtgcacggg
     3241 gagaggacgg acagaagata taaactctat ttaaaacata tatggaaaga aagaagataa
     3301 cattcgagtc gacggtatcg ataagcttga tatcgaattc ctgcagcccg ggggatccac
     3361 tagttctaga gcggccgcca ccgcggtgga ggggaattcc catgtcagcc gttaagtgtt
     3421 cctgtgtcac tcaaaattgc tttgagaggc tctaagggct tctcagtgcg ttacatccct
     3481 ggcttgttgt ccacaaccgt taaaccttaa aagctttaaa agccttatat attctttttt
     3541 ttcttataaa acttaaaacc ttagaggcta tttaagttgc tgatttatat taattttatt
     3601 gttcaaacat gagagcttag tacgtgaaac atgagagctt agtacgttag ccatgagagc
     3661 ttagtacgtt agccatgagg gtttagttcg ttaaacatga gagcttagta cgttaaacat
     3721 gagagcttag tacgtgaaac atgagagctt agtacgtact atcaacaggt tgaactgctg
     3781 atcttcagat cctctacgcc ggacgcatcg tggccggatc aattccgttt tccgctgcat
     3841 aaccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt ttcgcacgat
     3901 atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa cggcgtcagc
     3961 cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc actgtccctt
     4021 attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg gctaccgccg
     4081 gcgtaacaga tgagggcaag cggatggctg atgaaaccaa gccaaccagg aagggcagcc
     4141 cacctatcac ggaattgatc cccctcgaat tg
//
