LOCUS       sequence-121390-       13107 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13107)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 13107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB104
FEATURES             Location/Qualifiers
     source          1..13107
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    334..458
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(495..500,501..518,519..525)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             579..1238
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(645..664)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1580..1885
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1764..1783
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1926..2050)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2085..3896
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     terminator      3967..4219
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4253..4269)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4253..4269)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4266..4288)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4277..4293
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4301..4307,4308..4325,4326..4331))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4346..4367
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4452..4626
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4667..5692)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(5759..6434)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    6629..6653
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7174..7965
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8211..8799
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8700..8719
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8953..8970
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8985..9125
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9051..9070)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9211..9233
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9469..9663
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9729..10802)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11231..11860)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt cgactctaat caaacaagtt tgtacaaaaa agctgaacga
      361 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact
      421 acataatact gtaaaacaca acatatccag tcactatggc ggcccctcga ggggccgcat
      481 taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg attttgagtt
      541 aggatccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
      601 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
      661 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
      721 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
      781 atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg
      841 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
      901 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
      961 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
     1021 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     1081 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     1141 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat
     1201 tacaacagta ctgcgatgag tggcagggcg gggcgtaaag atctggatcc ggcttactaa
     1261 aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga atatatactg
     1321 atatgtatac ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta ttacagtgac
     1381 agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat ctccggtctg
     1441 gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa
     1501 aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac
     1561 gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag agagccgtta
     1621 tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac ggatggtgat
     1681 ccccctggcc agtgcacgtc tgctgtcaga taaagtcccc cgtgaacttt acccggtggt
     1741 gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc
     1801 cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat
     1861 taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg
     1921 tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca
     1981 aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt
     2041 acaaagtggt tcgatctaga ggatccccgg gtggtcagtc ccttatgtta cgtcctgtag
     2101 aaaccccaac ccgtgaaatc aaaaaactcg acggcctgtg ggcattcagt ctggatcgcg
     2161 aaaactgtgg aattgatcag cgttggtggg aaagcgcgtt acaagaaagc cgggcaattg
     2221 ctgtgccagg cagttttaac gatcagttcg ccgatgcaga tattcgtaat tatgcgggca
     2281 acgtctggta tcagcgcgaa gtctttatac cgaaaggttg ggcaggccag cgtatcgtgc
     2341 tgcgtttcga tgcggtcact cattacggca aagtgtgggt caataatcag gaagtgatgg
     2401 agcatcaggg cggctatacg ccatttgaag ccgatgtcac gccgtatgtt attgccggga
     2461 aaagtgtacg tatcaccgtt tgtgtgaaca acgaactgaa ctggcagact atcccgccgg
     2521 gaatggtgat taccgacgaa aacggcaaga aaaagcagtc ttacttccat gatttcttta
     2581 actatgccgg aatccatcgc agcgtaatgc tctacaccac gccgaacacc tgggtggacg
     2641 atatcaccgt ggtgacgcat gtcgcgcaag actgtaacca cgcgtctgtt gactggcagg
     2701 tggtggccaa tggtgatgtc agcgttgaac tgcgtgatgc ggatcaacag gtggttgcaa
     2761 ctggacaagg cactagcggg actttgcaag tggtgaatcc gcacctctgg caaccgggtg
     2821 aaggttatct ctatgaactg tgcgtcacag ccaaaagcca gacagagtgt gatatctacc
     2881 cgcttcgcgt cggcatccgg tcagtggcag tgaagggcga acagttcctg attaaccaca
     2941 aaccgttcta ctttactggc tttggtcgtc atgaagatgc ggacttgcgt ggcaaaggat
     3001 tcgataacgt gctgatggtg cacgaccacg cattaatgga ctggattggg gccaactcct
     3061 accgtacctc gcattaccct tacgctgaag agatgctcga ctgggcagat gaacatggca
     3121 tcgtggtgat tgatgaaact gctgctgtcg gctttaacct ctctttaggc attggtttcg
     3181 aagcgggcaa caagccgaaa gaactgtaca gcgaagaggc agtcaacggg gaaactcagc
     3241 aagcgcactt acaggcgatt aaagagctga tagcgcgtga caaaaaccac ccaagcgtgg
     3301 tgatgtggag tattgccaac gaaccggata cccgtccgca aggtgcacgg gaatatttcg
     3361 cgccactggc ggaagcaacg cgtaaactcg acccgacgcg tccgatcacc tgcgtcaatg
     3421 taatgttctg cgacgctcac accgatacca tcagcgatct ctttgatgtg ctgtgcctga
     3481 accgttatta cggatggtat gtccaaagcg gcgatttgga aacggcagag aaggtactgg
     3541 aaaaagaact tctggcctgg caggagaaac tgcatcagcc gattatcatc accgaatacg
     3601 gcgtggatac gttagccggg ctgcactcaa tgtacaccga catgtggagt gaagagtatc
     3661 agtgtgcatg gctggatatg tatcaccgcg tctttgatcg cgtcagcgcc gtcgtcggtg
     3721 aacaggtatg gaatttcgcc gattttgcga cctcgcaagg catattgcgc gttggcggta
     3781 acaagaaagg gatcttcact cgcgaccgca aaccgaagtc ggcggctttt ctgctgcaaa
     3841 aacgctggac tggcatgaac ttcggtgaaa aaccgcagca gggaggcaaa caatgaatca
     3901 acaactctcc tggcgcacca tcgtcggcta cagcctcggg aattgctacc gagctcgaat
     3961 ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     4021 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     4081 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     4141 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     4201 tcatctatgt tactagatcg ggaattggtt ccggaaccaa ttcgtaatca tggtcatagc
     4261 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     4321 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaggc tgaattaggc
     4381 gcgcctattt ctgaattcaa cacattgcgg acgtttttaa tgtactgaat taacgccgaa
     4441 ttaattcggg ggatctggat tttagtactg gattttggtt ttaggaatta gaaattttat
     4501 tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat
     4561 gagcgaaacc ctataggaac cctaattccc ttatctggga actactcaca cattattatg
     4621 gagaaactcg agcttgtcga tcgacagatc ccggtcggca tctactctat ttctttgccc
     4681 tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg
     4741 tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc
     4801 ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca
     4861 agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagt cgtggcgatc
     4921 ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc
     4981 acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc
     5041 tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat
     5101 ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agctcatcga
     5161 gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat
     5221 ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg
     5281 gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc gcatccatag
     5341 cctccgcgac cggttgtaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga
     5401 caccctgtga acggcgggag atgcaatagg tcaggctctc gctaaactcc ccaatgtcaa
     5461 gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa cgatctttgt
     5521 agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct acatcgaagc
     5581 tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg ctgtcgaact
     5641 tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc atatctcatt
     5701 gccccccgga tctgcgaaag ctcgagagag atagatttgt agagagagac tggtgatttc
     5761 agcgtgtcct ctccaaatga aatgaacttc cttatataga ggaaggtctt gcgaaggata
     5821 gtgggattgt gcgtcatccc ttacgtcagt ggagatatca catcaatcca cttgctttga
     5881 agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt
     5941 gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca
     6001 tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca
     6061 atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg
     6121 gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat
     6181 gttcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc
     6241 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag
     6301 cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt
     6361 tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt
     6421 gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt
     6481 agacgagagt gtcgtgctcc accatgttgg caagctgctc tagccaatac gcaaaccgaa
     6541 ttcagaaata aattcagcct aattcggcgt taattcagta cattaaaaac gtccgcaatg
     6601 tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca
     6661 gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat
     6721 cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac
     6781 cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg
     6841 gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc aattcgggca
     6901 cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc
     6961 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     7021 ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag
     7081 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
     7141 cagggcagtc gccctaaaac aaagttaaac atcatggggg aagcggtgat cgccgaagta
     7201 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg
     7261 gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat
     7321 ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac
     7381 cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc
     7441 attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt
     7501 ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt
     7561 gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg
     7621 gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc
     7681 ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg
     7741 ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc
     7801 gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag
     7861 gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt
     7921 gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct agctagaaat
     7981 tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca
     8041 cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca
     8101 taataagccc tacacaaatt gggagatata tcatgcatga ccaaaatccc ttaacgtgag
     8161 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     8221 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     8281 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     8341 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     8401 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     8461 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     8521 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     8581 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     8641 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     8701 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     8761 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     8821 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     8881 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     8941 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     9001 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     9061 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     9121 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     9181 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     9241 cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc
     9301 gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag
     9361 cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga
     9421 agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc
     9481 aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt
     9541 ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc
     9601 ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc
     9661 acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc
     9721 tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact
     9781 aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac
     9841 ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt
     9901 tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg
     9961 cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc
    10021 acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg
    10081 atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca
    10141 ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg
    10201 tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg
    10261 gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg
    10321 tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt
    10381 accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc
    10441 ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg
    10501 aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga
    10561 acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct
    10621 gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg
    10681 gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc
    10741 accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc
    10801 acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag acaacccagc
    10861 cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc
    10921 ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt
    10981 attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg
    11041 gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga
    11101 aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg
    11161 ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta
    11221 cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg
    11281 cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt
    11341 agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct
    11401 cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt
    11461 gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg
    11521 cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg
    11581 cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc
    11641 cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt
    11701 gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca
    11761 gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc
    11821 ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg
    11881 tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa
    11941 tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca
    12001 ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca cggttgagac
    12061 gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga tctcttcggt
    12121 aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt
    12181 tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg
    12241 cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc
    12301 gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc
    12361 agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg
    12421 cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg
    12481 aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt ggtggtgtcg
    12541 gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg ctcggcaatg
    12601 tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt cacaacgtcg
    12661 ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct ggtgccggtg
    12721 atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc
    12781 aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc ggcgctcttg
    12841 ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg actaaaacac
    12901 gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg
    12961 tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac tgcactatag
    13021 cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg ttggcatgca
    13081 catacaaatg gacgaacgga taaacct
//
