LOCUS       sequence-121400-        1171 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human EXO1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   El-Shemerly M, Janscak P, Hess D, Jiricny J, Ferrari S
  TITLE     Degradation of human exonuclease 1b upon DNA synthesis inhibition.
  JOURNAL   Cancer Res. 2005 May 1;65(9):3604-9.
  PUBMED    15867354
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Res."; date: "2005-05-1"; volume: "65(9)"; pages: "3604-9"
COMMENT     Sequence Label: pcDNA3.1 HisC EXO1b
FEATURES             Location/Qualifiers
     source          1..1171
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..81
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             131..148
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             152..184
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(188..196,197..211)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 cgggggcatt agcgagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa
       61 ttaatacgac tcactatagg gagacccaag ctggctagcg tttaaactta agcttaccat
      121 ggggggttct catcatcatc atcatcatgg tatggctagc atgactggtg gacagcaaat
      181 gggtcgggat ctgtacgacg atgacgataa ggtaccagga tccgggatac agggattgct
      241 acaatttatc aaagaagctt cagaacccat ccatgtgagg aagtataaag ggcaggtagt
      301 agctgtggat acatattgct ggcttcacaa aggagctatt gcttgtgctg aaaaactagc
      361 caaaggtgaa cctactgata ggtatgtagg attttgtatg aaatttgtaa atatgttact
      421 atctcatggg atcaagccta ttctcgtatt tgatggatgt actttacctt ctaaaaagga
      481 agtagagaga tctagaagag aaagacgaca agccaatctt cttaagggaa agcaacttct
      541 tcgtgagggg aaagtctcgg aagctcgaga gtgtttcacc cggtctatca atatcacaca
      601 tgccatggcc cacaaagtaa ttaaagctgc ccggtctcag ggggtagatt gcctcgtggc
      661 tccctatgaa gctgatgcgc agttggccta tcttaacaaa gcgggaattg tgcaagccat
      721 aattacagag gactcggatc tcctagcttt tggctgtaaa aaggtaattt taaagatgga
      781 ccagtttgga aatggacttg aaattgatca agctcggcta ggaatgtgca gacagcttgg
      841 ggatgtattc acggaagaga agtttcgtta catgtgtatt ctttcaggtt gtgactacct
      901 gtcatcactg cgtgggattg gattagcaaa ggcatgcaaa gtcctaagac tagccaataa
      961 tccagatata gtaaaggtta tcaagaaaat tggacattat ctcaagatga atatcacggt
     1021 accagaggat tacatcaacg ggtttattcg ggccaacaat accttcctct atcagctagt
     1081 ttttgatccc atcaaaagga aacttattcc tctgaacgcc tatgaagatg atgttgatcc
     1141 tgaaacacta agctacgctg ggcaatatgt t
//
