LOCUS       sequence-121404-        1082 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of Plk1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1082)
  TITLE     Stefano Ferrari plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTXB3-Flag PLK1
FEATURES             Location/Qualifiers
     source          1..1082
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             21..43
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             105..128
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcganattcc ctctgaatat tttgtttaac tttaagaagg agatataacc atggctagca
       61 tggactanta agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg
      121 atgacaagct tatgagtgct gcagtgactg cagggaagct ggcacgggca ccggccgacc
      181 ctgggaaagc cggggtcccc ggagttgcag ctcccggagc tccggcggcg gctccaccgg
      241 cgaaagagat cccggaggtc ctagtggacc cacgcagccg gcggcgctat gtgcggggcc
      301 gctttttggg caagggcggc tttgccaagt gcttcgagat ctcggacgcg gacaccaagg
      361 aggtgttcgc gggcaagatt gtgcctaagt ctctgctgct caagccgcac cagagggaga
      421 agatgtccat ggaaatatcc attcaccgca gcctcgccca ccagcacgtc gtaggattcc
      481 acggcttttt cgaggacaac gacttcgtgt tcgtggtgtt ggagctctgc cgccggaggt
      541 ctctcctgga gctgcacaag aggaggaaag ccctgactga gcctgaggcc cgatactacc
      601 tacggcaaat tgtgcttggc tgccagtacc tgcaccgaaa ccgagttatt catcgagacc
      661 tcaagctggg caaccttttc ctgaatgaag atctggaggt gaaaataggg gattttggac
      721 tggcaaccaa agtcgaatat gacggggaga ggaagaagac cctgtgtggg actcctaatt
      781 acatagctcc cgaggtgctg agcaagaaag ggcacagttt cgaggtggat gtgtggtcca
      841 ttgggtgtat catgtatacc ttgttagtgg gcaaaccacc ttttgagact tcttgcctaa
      901 aagagaccta cctccggatc aagaagaatg aatacagtat tcccaagcac atcaaccccg
      961 tggccgcctc cctcatccag aagatgcttc agacagatcc cactgcccgc ccaaccatta
     1021 acgagctgct taatgacgag ttctttactt ctggctatat ccctgcccgt ctccccatca
     1081 cc
//
