LOCUS       sequence-121410-        1101 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of Plk1 T210D mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1101)
  TITLE     Stefano Ferrari plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTXB3-Flag PLK1 T210D
FEATURES             Location/Qualifiers
     source          1..1101
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..121
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ttcctctaga atattttgtt tactttaaga aggagatata accatggcta gcatggacta
       61 caaagaccat gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa
      121 gcttatgagt gctgcagtga ctgcagggaa gctggcacgg gcaccggccg accctgggaa
      181 agccggggtc cccggagttg cagctcccgg agctccggcg gcggctccac cggcgaaaga
      241 gatcccggag gtcctagtgg acccacgcag ccggcggcgc tatgtgcggg gccgcttttt
      301 gggcaagggc ggctttgcca agtgcttcga gatctcggac gcggacacca aggaggtgtt
      361 cgcgggcaag attgtgccta agtctctgct gctcaagccg caccagaggg agaagatgtc
      421 catggaaata tccattcacc gcagcctcgc ccaccagcac gtcgtaggat tccacggctt
      481 tttcgaggac aacgacttcg tgttcgtggt gttggagctc tgccgccgga ggtctctcct
      541 ggagctgcac aagaggagga aagccctgac tgagcctgag gcccgatact acctacggca
      601 aattgtgctt ggctgccagt acctgcaccg aaaccgagtt attcatcgag acctcaagct
      661 gggcaacctt ttcctgaatg aagatctgga ggtgaaaata ggggattttg gactggcaac
      721 caaagtcgaa tatgacgggg agaggaagaa ggacctgtgt gggactccta attacatagc
      781 tcccgaggtg ctgagcaaga aagggcacag tttcgaggtg gatgtgtggt ccattgggtg
      841 tatcatgtat accttgttag tgggcaaacc accttttgag acttcttgcc taaaagagac
      901 ctacctccgg atcaagaaga atgaatacag tattcccaag cacatcaacc ccgtggccgc
      961 ctccctcatc cagaagatgc ttcagacaga tcccactgcc cgcccaacca ttaacgagct
     1021 gcttaatgac gagttcttta cttctggcta tatccctgcc cgtctcccca tcacctgcct
     1081 gaccattcca ccaaggtttt c
//
