LOCUS       sequence-121418-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, 
            mVenus-tagged RhoC Q63L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Zawistowski JS, Sabouri-Ghomi M, Danuser G, Hahn KM, Hodgson L
  TITLE     A RhoC biosensor reveals differences in the activation kinetics of 
            RhoA and RhoC in migrating cells.
  JOURNAL   PLoS One. 2013 Nov 5;8(11):e79877. doi: 
            10.1371/journal.pone.0079877. eCollection 2013.
  PUBMED    24224016
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-11-5"; volume: "8(11):e79877. doi"; pages: " 
            10.1371/journal.pone.0079877. eCollection 2013"
COMMENT     Sequence Label: RhoC FLARE.sc mCer, mVenus - CA
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     19..40
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             680..703
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(793..812)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
ORIGIN
        1 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
       61 cggcatggac gagctgtaca aggaattcac catggctgca atccgaaaga agctggtgat
      121 cgttggggat ggtgcctgtg ggaagacctg cctcctcatc gtcttcagca aggatcagtt
      181 tccggaggtc tacgtcccta ctgtctttga gaactatatt gcggacattg aggtggacgg
      241 caagcaggtg gagctggctc tgtgggacac agcagggctg gaagactatg atcgactgcg
      301 gcctctctcc tacccggaca ctgatgtcat cctcatgtgc ttctccatcg acagccctga
      361 cagcctggaa aacattcctg agaagtggac cccagaggtg aagcacttct gccccaacgt
      421 gcccatcatc ctggtgggga ataagaagga cctgaggcaa gacgagcaca ccaggagaga
      481 gctggccaag atgaagcagg agcccgttcg gtctgaggaa ggccgggaca tggcgaaccg
      541 gatcagtgcc tttggctacc ttgagtgctc agccaagacc aaggagggag tgcgggaggt
      601 gtttgagatg gccactcggg ctggcctcca ggtccgcaag aacaagcgtc ggaggggctg
      661 tcccattctc tgactcgagc accaccatca ccatcaccat cactaagtga ttaacctcag
      721 gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac
      781 cactgagatc gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga
      841 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
//
