LOCUS       sequence-121419-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for cell-free expression with C-term 
            sfGFP 8xHis tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Gagoski D, Mureev S, Giles N, Johnston W, Dahmer-Heath M, Skalamera 
            D, Gonda TJ, Alexandrov K
  TITLE     Gateway-compatible vectors for high-throughput protein expression in
            pro- and eukaryotic cell-free systems.
  JOURNAL   J Biotechnol. 2015 Feb 10;195:1-7. doi: 
            10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19.
  PUBMED    25529348
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Biotechnol."; date: "2015-02-10"; volume: "195:1-7. doi"; pages: " 
            10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19"
COMMENT     Sequence Label: pCellFree_G10
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(167..190)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(231..251)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga gagatcatac
       61 atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct acaaccgaga
      121 aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttagtgg tgatggtggt
      181 gatggtggtg tttgtagagc tcatccatgc catgtgtaat cccagcagca gttacaaact
      241 caagaaggac catgtggtca cgcttttcgt tgggatcttt cgaaaggaca gattgtgtcg
      301 acaggtaatg gttgtctggt aaaaggacag ggccatcgcc aattggagta ttttgttgat
      361 aatggtctgc tagttgaacg gaaccatctt caacgttgtg gcgaattttg aagttagctt
      421 tgattccatt cttttgtttg tctgccgtga tgtatacatt gtgtgagtta aagttgtact
      481 cgagtttgtg tccaagaatg tttccatctt ctttaaaatc aatacccttt aactcgatac
      541 gattaacaag ggtatcacct tcaaacttga cttcagcacg cgtcttgtag gtcccgtcat
      601 ctttgaaaga tatagtgcgt tcctgtacat aaccttcggg catggcactc ttgaaaaagt
      661 catgccgttt catgtgatcc ggataacggg aaaagcattg aacaccatag gtcagagtag
      721 tgacaagtgt tggccacgga acaggtagtt ttccagtagt gcaaataaat ttaagggtga
      781 gttttccgtt tgtagcatca cctttcaccc tctccacgga cagaaaaatt tgtgcccatt
      841 aaacatcacc atctaattca acaagaa
//
