LOCUS       sequence-121422-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  G protein alpha-s internally tagged with ECFP and EE epitope.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Hynes TR, Mervine SM, Yost EA, Sabo JL, Berlot CH
  TITLE     Live cell imaging of Gs and the beta2-adrenergic receptor 
            demonstrates that both alphas and beta1gamma7 internalize upon 
            stimulation and exhibit similar trafficking patterns that differ 
            from that of the beta2-adrenergic receptor.
  JOURNAL   J Biol Chem. 2004 Oct 15;279(42):44101-12. Epub 2004 Aug 5.
  PUBMED    15297467
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-10-15"; volume: "279(42)"; pages: "44101-12. 
            Epub 2004 Aug 5"
COMMENT     Sequence Label: G protein alpha-s-CFP
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     213..234
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      510..519
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(722..741)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(723..741)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(786..837)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(815..835)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
       61 gctgaagcac tgcacgcccc aggtcagggt ggtcacgagg gtgggccagg gcacgggcag
      121 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc
      181 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
      241 caccaccccg gtgaacagct cctcgccctt gctcaccatg ctcccgcccc caccggatcc
      301 ctctccgtta aacccattaa catgtagaat cctcatctgc ttcacaatgg tgcttttgcc
      361 agactctcca gcacccagca gcagcaggcg gtgcgtggcc cggtagacct gcttgtcctt
      421 ctgcagctgc ttctcgatct ttttgttggc ctcgcgctgc gccttctcct cgttgcgctg
      481 gtcctcggtc ttactgttgc cgaggcagcc catggcggcg gcggggcgcg gccgggctgc
      541 ggggcggcgg ggacggggcg ggcggcctcg ggcgggccgg gaaagcgggg aacgcgggga
      601 gggtggcggc tcggactaag gcaagcgcgc ggctctccgc ccctcgctcc ggggccgagg
      661 cggagcagag caggagccgc gggagctgct gctgcggaat tggtcgacca agcttgggtc
      721 tccctatagt gagtcgtatt aatttcgata agccagtaag cagtgggttc tctagttagc
      781 cagagagctc tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat
      841 gggg
//
