LOCUS       sequence-121425-        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for Paph-mCherry integration downstream of B. diazoefficiens
            (B. japonicum) scoI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Ledermann R, Bartsch I, Remus-Emsermann M, Vorholt JA, Fischer HM
  TITLE     Stable fluorescent and enzymatic tagging of Bradyrhizobium 
            diazoefficiens to analyze host-plant infection and colonization.
  JOURNAL   Mol Plant Microbe Interact. 2015 Jun 2.
  PUBMED    26035130
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant 
            Microbe Interact. 2015 Jun 2."
COMMENT     Sequence Label: pRJPaph-mChe
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(657..675)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     925..944
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
       61 agctcaccgt ctttcattgc catacggaat tcgagctcgc acgctgccgc aagcactcag
      121 ggcgcaaggg ctgctaaagg aagcggaaca cgtagaaagc cagtccgcag aaacggtgct
      181 gaccccggat gaatgtcagc tactgggcta tctggacaag ggaaaacgca agcgcaaaga
      241 gaaagcaggt agcttgcagt gggcttacat ggcgatagct agactgggcg gttttatgga
      301 cagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg aagccctgca
      361 aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga tcaagatctg
      421 atcaagagac aggatactag tggatccccc gggctgcagg aattcgatat caagcttaga
      481 tcttgactag aaataagaag gagatataat tatggtgagc aagggcgagg aggataacat
      541 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
      601 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
      661 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
      721 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
      781 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
      841 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
      901 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
      961 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     1021 gaa
//
