LOCUS       sequence-121447-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses destabilized Firefly Luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Seo GJ, Fink LH, O'Hara B, Atwood WJ, Sullivan CS
  TITLE     Evolutionarily conserved function of a viral microRNA.
  JOURNAL   J Virol. 2008 Oct;82(20):9823-8. doi: 10.1128/JVI.01144-08. Epub 
            2008 Aug 6.
  PUBMED    18684810
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.01144-08"; journalName: "J Virol"; date: "2008-10"; 
            volume: "82"; issue: "20"; pages: "9823-8"
COMMENT     Sequence Label: pcDNA3.1dsLuc2CP
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             62..661
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(62..81)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     558..578
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg gggccgccac
       61 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt
      121 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgacccgga
      181 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
      241 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
      301 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
      361 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
      421 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
      481 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
      541 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
      601 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
      661 attcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
      721 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
      781 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
      841 taatggttac aaataaagca atagca
//
