LOCUS       sequence-121450-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses destabilized Renilla Luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Seo GJ, Fink LH, O'Hara B, Atwood WJ, Sullivan CS
  TITLE     Evolutionarily conserved function of a viral microRNA.
  JOURNAL   J Virol. 2008 Oct;82(20):9823-8. doi: 10.1128/JVI.01144-08. Epub 
            2008 Aug 6.
  PUBMED    18684810
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.01144-08"; journalName: "J Virol"; date: "2008-10"; 
            volume: "82"; issue: "20"; pages: "9823-8"
COMMENT     Sequence Label: pcDNA3.1dsRluc
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             64..663
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(64..83)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     560..580
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(860..879)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 agagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cggggccgcc
       61 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc
      121 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg
      181 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
      241 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
      301 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
      361 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
      421 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
      481 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
      541 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
      601 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
      661 tgattcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
      721 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
      781 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
      841 tataatggtt acaaataaag caatagcatc acaaatttca caaataaa
//
