LOCUS       sequence-121452-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for Paph-lacZYA integration downstream of B. diazoefficiens 
            (B. japonicum) scoI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Ledermann R, Bartsch I, Remus-Emsermann M, Vorholt JA, Fischer HM
  TITLE     Stable fluorescent and enzymatic tagging of Bradyrhizobium 
            diazoefficiens to analyze host-plant infection and colonization.
  JOURNAL   Mol Plant Microbe Interact. 2015 Jun 2.
  PUBMED    26035130
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant 
            Microbe Interact. 2015 Jun 2."
COMMENT     Sequence Label: pRJPaph-lacZYA
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     162..184
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(209..225)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(209..225)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(222..244)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    233..249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cggcaccgct tctggtgccg gaaaccaggc aaagcgccat tcgccattca ggctgcgcaa
       61 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
      121 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
      181 aacgacggga tcgcaagctt gaatcatggt catagctgtt tcctgtgtga aattgttatc
      241 cgctcacaat tctgcacact agtatcctgt ctcttgatca gatcttgatc ccctgcgcca
      301 tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc
      361 agagggcgcc ccagctggca attccggttc gcttgctgtc cataaaaccg cccagtctag
      421 ctatcgccat gtaagcccac tgcaagctac ctgctttctc tttgcgcttg cgttttccct
      481 tgtccagata gcccagtagc tgacattcat ccggggtcag caccgtttct gcggactggc
      541 tttctacgtg ttccgcttcc tttagcagcc cttgcgccct gagtgcttgc ggcagcgtgc
      601 gagctcgaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
      661 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
      721 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
      781 aacctggcct atttccctaa aggg
//
