LOCUS       sequence-121454-         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses BLV (NC001414) B4 pre-miRNA and miRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Burke JM, Bass CR, Kincaid RP, Sullivan CS
  TITLE     Identification of tri-phosphatase activity in the biogenesis of 
            retroviral microRNAs and RNAP III-generated shRNAs.
  JOURNAL   Nucleic Acids Res. 2014 Dec 16;42(22):13949-62. doi: 
            10.1093/nar/gku1247. Epub 2014 Nov 26.
  PUBMED    25428356
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku1247"; journalName: "Nucleic Acids Res"; date: 
            "2014-12-16- 16"; volume: "42"; issue: "22"; pages: "13949-62"
COMMENT     Sequence Label: pBLV B4
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(253..269)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(253..269)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      317..348
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        349..451
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(525..544)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aggtgagcca gtgagttgat tgcagtccag ttacgctgga gtctgaggct cgtcctgaat
       61 gatatgcggc ctcctcgagc tgcagctgtg ctaagcgaga ggctctggtg ctggggataa
      121 gatgcggccc ctagcaccac agtctctgcg ccttttgggt tcgaatcttc cccatgcctc
      181 tagagcgcgt gatcttacgg cattatacgt atgatcggtc cacgatcagc tagattatct
      241 agtcagcttg atgtcatagc tgtttcctga ggctcaatac tgaccattta aatcatacct
      301 gacctccata gcagaaagtc aaaagcctcc gaccggaggc ttttgacttg atcggcacgt
      361 aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt
      421 atcgagattt tcaggagcta aggaagctaa aatgagccat attcaacggg aaacgtcttg
      481 cttgaagccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg
      541 cgataatgtc gggcaatcag gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc
      601 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt
      661 caggctaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac
      721 tcctgatgat gcatggttac tcaccactgc gatcccaggg aaaacagcat tccaggtatt
      781 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg
      841 gttgcattcg attcctgttt gtaattgtcc ttttaacggc gatcgcgtat ttcgtctcgc
      901 tcaggcgcaa tcacgaatga ataacggttt ggttggtgcg agtgatttga tgac
//
