LOCUS       sequence-121461-         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Guo W, Keckesova Z, Donaher JL, Shibue T, Tischler V, Reinhardt F, 
            Itzkovitz S, Noske A, Zurrer-Hardi U, Bell G, Tam WL, Mani SA, van 
            Oudenaarden A, Weinberg RA
  TITLE     Slug and Sox9 cooperatively determine the mammary stem cell state.
  JOURNAL   Cell. 2012 Mar 2;148(5):1015-28.
  PUBMED    22385965
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2012-03-2"; volume: "148(5)"; pages: "1015-28"
COMMENT     Sequence Label: FUW-Sox10
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             89..100
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     498..518
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(714..734)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gggagatccg actcgtctga gggcgaaggc gaagacgcgg aagaggccgc agagccggca
       61 gcaggcctcg ggaaggaagg tccgctggat tgagggccga agggacgtag cagaaggacg
      121 tcccgcgcag aatccaggtg gcaacacagg cgagcagcca aggaaaggac gatgatttcc
      181 ccgacaacac cacggaattg tcagtgccca acagccgagc ccctgtccag cagcgggcaa
      241 ggcaggcggc gatgagttcc gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa
      301 ggagctgaca ggtggtggca atgccccaac cagtgggggt tgcgtcagca aacacagtgc
      361 acaccacgcc acgttgcctg acaacgggcc acaactcctc ataaagagac agcaaccagg
      421 atttatacaa ggaggagaaa atgaaagcca tacgggaagc aatagcatga tacaaaggca
      481 ttaaagcagc gtatccacat agcgtaaaag gagcaacata gttaagaata ccagtcaatc
      541 tttcacaaat tttgtaatcc agaggttgat tatcgatgca tggggtcgtg cgctcctttc
      601 ggtcgggcgc tgcgggtcgt ggggcgggcg tcaggcaccg ggcttgcggg tcatgcacca
      661 ggtgcgcggt ccttcgggca cctcgacgtc ggcggtgacg gtgaagccga gccgctcgta
      721 gaaggggagg ttgcggggcg cggaggtctc caggaaggcg ggcaccccgg cgcgctcggc
      781 cgcctccact ccggggagca cgacggcgct gcccagaccc ttgccctggt ggtcgggcga
      841 gacgccgacg gtggccagga accacgcggg ctccttgggc cggtgcggcg ccag
//
