LOCUS       sequence-121463-        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for Paph-sYFP2 integration downstream of B. diazoefficiens 
            (B. japonicum) scoI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Ledermann R, Bartsch I, Remus-Emsermann M, Vorholt JA, Fischer HM
  TITLE     Stable fluorescent and enzymatic tagging of Bradyrhizobium 
            diazoefficiens to analyze host-plant infection and colonization.
  JOURNAL   Mol Plant Microbe Interact. 2015 Jun 2.
  PUBMED    26035130
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant 
            Microbe Interact. 2015 Jun 2."
COMMENT     Sequence Label: pRJPaph-sYFP2
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(448..450,451..453,454..966)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(493..514)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(754..773)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
       61 agctcaccgt ctttcattgc catacggaat tcgagctcgc acgctgccgc aagcactcag
      121 ggcgcaaggg ctgctaaagg aagcggaaca cgtagaaagc cagtccgcag aaacggtgct
      181 gaccccggat gaatgtcagc tactgggcta tctggacaag ggaaaacgca agcgcaaaga
      241 gaaagcaggt agcttgcagt gggcttacat ggcgatagct agactgggcg gttttatgga
      301 cagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg aagccctgca
      361 aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga tcaagatctt
      421 gactagaaat aagaaggaga tataattatg gtgagcaagg gcgaggagct gttcaccggg
      481 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      541 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc
      601 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg cgtgcagtgc
      661 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      721 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      781 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      841 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
      901 tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
      961 atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     1021 ggccccgtgc tgctgcccga caaccactac
//
