LOCUS       sequence-121466-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  inverted GFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Li Y, Park A, Mou H, Colpan C, Bizhanova A, Akama-Garren E, Joshi N,
            Hendrickson EA, Feldser D, Yin H, Anderson DG, Jacks T, Weng Z, Xue 
            W
  TITLE     A versatile reporter system for CRISPR-mediated chromosomal 
            rearrangements.
  JOURNAL   Genome Biol. 2015 May 28;16(1):111.
  PUBMED    26018130
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-05-28"; volume: "16(1)"; pages: "111"
COMMENT     Sequence Label: iGFP
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     192..209
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    326..347
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(362..367,368..385,386..392)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    400..416
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     405..427
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     424..440
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     424..440
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    489..610
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(500..519)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     554..573
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
       61 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
      121 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
      181 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
      241 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
      301 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
      361 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
      421 acacaggaaa cagctatgac catgattacg ccaagctcta gctagaggtc gacggtatac
      481 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
      541 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
      601 taaacaagtt ggggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc
      661 agccggcgtc ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat
      721 cgaaatctcg tagtacgtgc tattcctttg ccctcggacg agtgctgggg cgtcggtttc
//
