LOCUS       sequence-121476-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: S1PR3-Tango
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctgg ctagcgttta aacttaagct tggtaccgag ctcggatcca
      121 ctagtccagt gtggtggaat tctgcagata tccagcacag tggcggccgc gccaccatga
      181 agacgatcat cgccctgagc tacatcttct gcctggtatt cgccgactac aaggacgatg
      241 atgacgccag catcgatatg gcaaccgccc ttccgcccag gctccagcca gtgagaggta
      301 acgagaccct gagagagcac tatcagtatg ttggcaagct tgccggtaga ttgaaagagg
      361 catcagaggg atcaacactg acaaccgtcc tctttctcgt gatatgctct ttcatcgttc
      421 tggaaaatct gatggtcctg attgccattt ggaaaaacaa caagttccat aaccgaatgt
      481 attttttcat aggcaacctg gccctgtgcg acctgctcgc tggtatcgcc tataaagtga
      541 acatcctgat gagcggaaaa aagaccttta gtctgagccc aacagtgtgg ttcctgagag
      601 aagggagcat gttcgtggcg ctgggggcga gtacctgtag tcttctcgcc atcgcgattg
      661 aaagacactt gaccatgatt aagatgaggc catatgacgc aaacaagcga catcgggtgt
      721 ttctccttat cgggatgtgt tggctgatcg cattcactct cggggctctg cctatactgg
      781 ggtggaactg cctgcacaat ctcccagatt gctctacaat cctcccactg tatagtaaga
      841 aatatattgc attttgcatc tccatcttca ctgccatcct ggttaccatc gtcatcctgt
      901 acgcgcggat ctactttctg gtcaaaagta
//
