LOCUS       sequence-121490-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for Paph-sYFP2 integration downstream of B. diazoefficiens 
            (B. japonicum) scoI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Ledermann R, Bartsch I, Remus-Emsermann M, Vorholt JA, Fischer HM
  TITLE     Stable fluorescent and enzymatic tagging of Bradyrhizobium 
            diazoefficiens to analyze host-plant infection and colonization.
  JOURNAL   Mol Plant Microbe Interact. 2015 Jun 2.
  PUBMED    26035130
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant 
            Microbe Interact. 2015 Jun 2."
COMMENT     Sequence Label: pRJPaph-sYFP2
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             125..376
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     313..334
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
       61 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcac
      121 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga
      181 cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
      241 gctgctgccc gacaaccact acctgagcta ccagtccaag ctgagcaaag accccaacga
      301 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
      361 ggacgagctg tacaagtaag gtaccgacgt cttgcaggcg ttatctgtag tttggtggat
      421 cggtcggacc gcgtcctcgg tgtaacctct cccgcttgcg ggagaggtat aggccgcttt
      481 tggcggccgt cctctaagac gccgaggcaa agcctcggct atggcagagc gccgggtgag
      541 ggctctctcc tctcggggat tgtccctccg cggagacacc ctctccccgc ccccctcccg
      601 caagcgggag agggagcgca ccttcaccct gaccgcaatc ggcgctcgcg ttcccgggcg
      661 gatggtctat aaggccctcc gatcccaacg aaattcgttc atttccggcc gatggctcca
      721 tcgcaacagg cgaaatcgtc caaatctgcc cgtggcttgc tgacagggct ggccgtcggc
      781 gcctgcctgt tcgccggctt gcccggcgcg caggcgcagg ccccggccaa gcagcctccg
      841 gcggcgcccc aggtcacccc gcaggtcgcg ccccaggcgg ccccgcaggc cgtgcccggc
      901 ttctgggacc cgcggcggcg gccgga
//
