LOCUS       sequence-12150-e        3820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Karginov AV, Ding F, Kota P, Dokholyan NV, Hahn KM
  TITLE     Engineered allosteric activation of kinases in living cells.
  JOURNAL   Nat Biotechnol. 2010 Jul;28(7):743-7. doi: 10.1038/nbt.1639. Epub 
            2010 Jun 27.
  PUBMED    20581846
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.1639"; 
            journalName: "Nat Biotechnol"; date: "2010-07"; volume: "28"; issue:
            "7"; pages: "743-7"
COMMENT     Sequence Label: myc-Rapr-FAK-YM
FEATURES             Location/Qualifiers
     source          1..3820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..42
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        23..41
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      154..163
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             163..192
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
       61 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttctgcagat atccatcaca
      121 ctggcggccc cctcgaggtc gacgattgaa ttggccacca tggagcagaa gctgatctcc
      181 gaggaggacc tgggatcccc tgcttatctt gacccaaact tgaatcacac accaagttcg
      241 agtactaaga ctcacctggg tactggcatg gaacggtccc ctggtgcaat ggaacgagta
      301 ttaaaggtct ttcatcattt tgaaagcagt agtgagccaa ccacctgggc cagtattatc
      361 aggcatggag atgctactga tgtcaggggc atcattcaga agatagtgga cagtcacaaa
      421 gtaaagcacg tggcctgcta tggatttcgc ctcagtcacc tgcgatcgga ggaggtgcac
      481 tggctgcacg tggacatggg tgtctccagt gtgagggaga aatatgaact cgcccaccca
      541 ccagaggagt ggaaatatga gttgagaatt cgttacttgc caaaaggatt tctaaaccag
      601 tttactgaag ataagccaac attgaatttc ttctatcaac aggtgaagag tgactacatg
      661 caagaaatag ctgatcaagt agaccaagaa atagctttga agttgggttg tttggaaatt
      721 aggcgatcct attgggagat gaggggtaat gccctagaga agaagtccaa ctatgaagta
      781 ttagaaaaag atgttggttt aaagcgattt tttcctaaga gtttactgga ttctgtcaag
      841 gccaaaacac taagaaagct gatccagcaa acattcaggc agtttgccaa ccttaataga
      901 gaagaaagca tcctgaaatt ctttgagatt ctttctcctg tgtacagatt tgacaaagag
      961 tgcttcaagt gtgcccttgg gtcaagttgg atcatttctg tggaattggc aatcggccca
     1021 gaagaaggga tcagttacct gacagacaaa ggctgcaatc ccacacacct agcagacttt
     1081 aaccaagtgc agaccatcca gtactcaaac agtgaagaca aagacaggaa aggaatgcta
     1141 caactcaaaa tcgctggtgc tcctgagcct ctgaccgtga cggcaccatc cctaaccatt
     1201 gccgagaaca tggccgacct gattgatgga tactgccggc tggtgaatgg agccacacaa
     1261 tctttcatca tcagacccca gaaagaaggt gaacgggcgt tgccatcaat accaaagttg
     1321 gccaacagtg aaaagcaagg catgcggaca catgcagtct ctgtgtcaga gacagatgac
     1381 tatgcagaga tcatcgatga ggaagacaca tacaccatgc cctcgaccag ggattatgag
     1441 attcagagag aaagaataga acttggacgc tgtattggag aaggtcagtt tggagatgta
     1501 catcaaggcg tgtacggccc aggaacctgc gtggtgcact acaccgggat gcttgaagat
     1561 ggaaagaaat ttgattcctc ccgggacaga aacaagccct ttaagtttat gctaggcaag
     1621 caggaggtga tccgaggctg ggaagaaggg gttgcccaga tgagtgtggg tcagagagcc
     1681 aaactgacta tatctccaga ttatgcctat ggtgccactg ggcacccagg catcatccca
     1741 ccacatgcca ctctcgtctt cgatgtggag cttctaaaac tggaaggccc aggtttggct
     1801 gttgcaatca aaacatgtaa aaactgtact tcggacagcg tgagagagaa gttccttcaa
     1861 gaagccttaa caatgcgtca gtttgaccat cctcacatcg tgaagctgat tggagtcatt
     1921 acagagaacc ctgtctggat aatcatggag ttgtgcacac ttggagagct gaggtcattt
     1981 ttgcaagtaa gaaaatacag cttggacctg gcatctttga tattatatgc ctatcagctt
     2041 agtacagcac tcgcgtatct ggagagcaaa agatttgttc acagggacat tgctgctcgg
     2101 aatgttctgg tgtcttcaaa tgattgtgta aaattgggag actttggatt atctcgatat
     2161 atggaagaca gtacttacta taaagcttcc aaaggaaaat tacctattaa atggatggct
     2221 ccagagtcaa tcaattttcg acgttttacc tcagctagtg acgtgtggat gtttggtgtg
     2281 tgtatgtggg agatactgat gcatggcgtg aagccttttc aaggagtgaa gaacaatgat
     2341 gtgatcggtc gaattgaaaa tggggaaaga ttaccaatgc ctccaaattg tcctcccacc
     2401 ctctacagcc ttatgacgaa atgttgggcc tatgacccca gcaggcggcc caggtttact
     2461 gaactaaaag ctcagctcag cacaatcctg gaggaggaga aggtgcagca agaagaacgg
     2521 atgaggatgg aatccagaag acaggctacc gtgtcctggg actctggagg gtctgatgaa
     2581 gcaccaccca agcccagcag acctggttat cctagcccga gatccagtga aggattttat
     2641 cccagcccac agcacatggt ccagaccaat cactaccagg tctctggcta cccgggttcc
     2701 catggaatcc cagccatggc tggcagcatt taccaaggtc aggcatctct tctggaccag
     2761 acagaattgt ggaatcacag acctcaggag atgtccatgt ggcagcccag tgtggaggat
     2821 tcggcagctt tggaccttcg agggatggga caggtgcttc cccctcacct gatggaagag
     2881 cggctaatcc gacagcagca ggaaatggaa gaagaccagc gctggctgga gaaggaagag
     2941 cgattcctga aacctgatgt gaggctctct cgaggcagca tcgacaggga agacgggagt
     3001 tttcagggtc cgactggaaa ccaacacatc tatcagcctg tggggaagcc agatcctgca
     3061 gctccaccaa agaaacctcc tcgccctgga gcacctggcc acctaagcaa cctgtccagc
     3121 atcagcagcc ctgcagacag ctacaatgag ggtgtcaagc ttcagcccca ggaaatcagc
     3181 ccccctccca ctgccaacct tgaccggtcc aatgacaagg tatatgagaa tgtgacaggc
     3241 ctagtgaagg ctgtcatcga gatgtccagc aaaatccagc cagctcctcc agaagagtac
     3301 gtccctatgg tgaaggaagt tggcctggcc ctgcggacct tactagccac ggtggatgag
     3361 accattcctg ctcttccagc cagcactcat cgagagatcg agatggcaca gaagctgctg
     3421 aactccgact taggcgagct catcagcaag atgaagctgg cgcagcagta cgtcatgacc
     3481 agcctgcagc aggagtataa gaagcagatg ctgacagctg ctcacgccct ggctgtggat
     3541 gccaagaacc tacttgatgt tattgatcaa gcaagactga aaatgctagg gcagacacgg
     3601 ccacactgag cccctggcca ttgaacgtgg ctttctgccc tttgggagat cttctctagc
     3661 attccaccag cagcgaggaa gtaaccttag cctctgtagc ccgtgctcac ggctccaact
     3721 gaatgacagc tagctgaaag tctctcttgt ataagtttca tcacttatac atggttttga
     3781 ttttgttatt tttctaaata gtgttcagaa aactctggat
//
