LOCUS       sequence-121504-       12571 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12571)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 12571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB405
FEATURES             Location/Qualifiers
     source          1..12571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(1178..1180,1181..1183,1184..1894)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1223..1244)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1484..1503)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1831..1852
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1904..2028
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2065..2070,2071..2088,2089..2095)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2149..2808
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2215..2234)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3150..3455
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3334..3353
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3496..3620)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3646..3898
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3932..3948)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3932..3948)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3945..3967)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3956..3972
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3980..3986,3987..4004,4005..4010))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4025..4046
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4131..4305
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4362..5159)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4473..4492)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5083..5102
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5222..5898)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    6093..6117
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6638..7429
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7675..8263
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8164..8183
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8417..8434
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8449..8589
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8515..8534)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8675..8697
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8933..9127
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9193..10266)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10695..11324)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcatg gtgagcaagg gcgaggagct
     1201 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1261 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1321 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg
     1381 cctgatgtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1441 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1501 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1561 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1621 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1681 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1741 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct
     1801 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1861 cgggatcact ctcggcatgg acgagctgta caaggctgtt atcacaagtt tgtacaaaaa
     1921 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
     1981 aaaacagact acataatact gtaaaacaca acatatccag tcactatggc ggcccctcga
     2041 ggggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg
     2101 attttgagtt aggatccggc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
     2161 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
     2221 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
     2281 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
     2341 cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata
     2401 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
     2461 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
     2521 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc
     2581 gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac
     2641 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
     2701 atgccgctgg cgattcaggt tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg
     2761 cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaaac gcgtggatcc
     2821 ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga
     2881 atatatactg atatgtatac ccgaagtatg tcaaaaagag gtgtgctatg aagcagcgta
     2941 ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat
     3001 ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg
     3061 gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc
     3121 ttttgctgac gagaacaggg actggtgaaa tgcagtttaa ggtttacacc tataaaagag
     3181 agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac
     3241 ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt
     3301 acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg
     3361 tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca
     3421 aaaacgccat taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc
     3481 agtctgcagg tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt
     3541 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca
     3601 gctttcttgt acaaagtggt gataacctag agctcgaatt tccccgatcg ttcaaacatt
     3661 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
     3721 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
     3781 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
     3841 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg
     3901 gaattggttc cggaaccaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     3961 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     4021 cctaatgagt gagctaactc acattaggct gaattaggcg cgcctatttc tgaattcaac
     4081 acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcgggg gatctggatt
     4141 ttagtactgg attttggttt taggaattag aaattttatt gatagaagta ttttacaaat
     4201 acaaatacat actaagggtt tcttatatgc tcaacacatg agcgaaaccc tataggaacc
     4261 ctaattccct tatctgggaa ctactcacac attattatgg agaaactcga gcttgtcgat
     4321 cgactctagc tagaggatcg atccgaaccc cagagtcccg ctcagaagaa ctcgtcaaga
     4381 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag
     4441 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc
     4501 tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt
     4561 tccaccatga tattcggcaa gcaggcatcg ccatgtgtca cgacgagatc ctcgccgtcg
     4621 ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg
     4681 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga
     4741 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt
     4801 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc
     4861 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca
     4921 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctggagt
     4981 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac
     5041 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat
     5101 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatccccatg
     5161 gtcgatcgac agatctgcga aagctcgaga gagatagatt tgtagagaga gactggtgat
     5221 ttcagcgtgt cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag
     5281 gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct
     5341 ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat
     5401 ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat
     5461 ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg
     5521 ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc
     5581 tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca
     5641 ccatgttcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg
     5701 atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg
     5761 atagcctttc ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc
     5821 cttttgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc
     5881 ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg
     5941 gagtagacga gagtgtcgtg ctccaccatg ttggcaagct gctctagcca atacgcaaac
     6001 cgaattcaga aataaattca gcctaattcg gcgttaattc agtacattaa aaacgtccgc
     6061 aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca
     6121 gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc
     6181 ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg
     6241 ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct
     6301 cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaattcg
     6361 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     6421 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     6481 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     6541 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     6601 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga
     6661 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     6721 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     6781 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     6841 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     6901 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     6961 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     7021 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     7081 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     7141 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     7201 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     7261 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     7321 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     7381 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctagctag
     7441 aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta
     7501 agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg
     7561 tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa tcccttaacg
     7621 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     7681 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     7741 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     7801 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     7861 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     7921 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     7981 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     8041 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     8101 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     8161 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     8221 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     8281 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     8341 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     8401 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     8461 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     8521 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     8581 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     8641 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     8701 tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag
     8761 tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt
     8821 tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga
     8881 ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca
     8941 ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc
     9001 ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg
     9061 gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct
     9121 atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt gccctagcat
     9181 ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat
     9241 gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt
     9301 tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact
     9361 gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc
     9421 caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt
     9481 cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat
     9541 ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc
     9601 ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc
     9661 aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc
     9721 atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg
     9781 cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat
     9841 cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac
     9901 gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag
     9961 acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat
    10021 cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc
    10081 ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc
    10141 accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt
    10201 ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc
    10261 gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc
    10321 cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg
    10381 gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc
    10441 atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt
    10501 aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa
    10561 ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt
    10621 gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc
    10681 tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg
    10741 cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct
    10801 gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc
    10861 gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc
    10921 gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg
    10981 gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg
    11041 atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc
    11101 cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc
    11161 ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact
    11221 aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc
    11281 ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta
    11341 tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact
    11401 caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg
    11461 gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg
    11521 agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt
    11581 cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg
    11641 gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc
    11701 accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag
    11761 ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc
    11821 gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt
    11881 cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg
    11941 ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt
    12001 gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc
    12061 aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac
    12121 gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc
    12181 ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt
    12241 ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct
    12301 cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa
    12361 acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga
    12421 cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact
    12481 atagcagcgg aggggttgga tcaaagtact ttgatcccga ggggaaccct gtggttggca
    12541 tgcacataca aatggacgaa cggataaacc t
//
