LOCUS       sequence-121512-        1088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: TSHR-Tango
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctgg ctagcgttta aacttaagct tggtaccgag ctcggatcca
      121 ctagtccagt gtggtggaat tctgcagata tccagcacag tggcggccgc gccaccatga
      181 agacgatcat cgccctgagc tacatcttct gcctggtatt cgccgactac aaggacgatg
      241 atgacgccag catcgatatg cgccctgccg atctgctgca gctggtgctt ctccttgacc
      301 ttccccgcga cttggggggc atggggtgca gctcaccccc ttgtgaatgt catcaggagg
      361 aagactttcg agtgacctgc aaagacattc agagaatccc atcactcccc ccaagcacac
      421 agactctgaa gctgatcgaa acccatctgc ggacaattcc tagccacgcc ttctcaaatc
      481 ttcccaacat ttctcgcata tacgtgtcaa tcgacgtaac gcttcagcag ttggaatctc
      541 attccttcta taatctgagc aaggtgaccc acattgaaat tcggaacact aggaacctca
      601 cgtacataga tcccgacgca cttaaggaac tgccattgct gaagttcctt ggcattttca
      661 atactggact gaagatgttt ccagacctga ccaaggtgta ctccaccgac atattcttta
      721 ttttggagat caccgataac ccttatatga caagtattcc tgttaacgct tttcaggggt
      781 tgtgcaacga aaccctgacg ctcaagttgt acaataacgg gttcaccagc gtacagggtt
      841 acgccttcaa tgggaccaag ttggacgccg tctacctcaa taagaacaag tatctcactg
      901 ttattgataa ggatgcattc ggcggggtgt actcaggccc ctccctcctg gatgttagcc
      961 agacaagtgt caccgcattg cccagtaagg gcctggagca cctgaaagag ctcatcgcac
     1021 gcaatacatg gaccctgaag aagctcccgc tttctttgtc ctttctgcat ctgaccaggg
     1081 ctgatcgt
//
