LOCUS       sequence-121518-         371 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This is a BAC vector that contains both wt and mutant loxP sites.  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Coren JS
  TITLE     Retrofitting the BAC cloning vector pBeloBAC11 by the insertion of a
            mutant loxP site.
  JOURNAL   BMC Res Notes. 2017 Jul 28;10(1):344. doi: 
            10.1186/s13104-017-2631-8.
  PUBMED    28754135
REFERENCE   2  (bases 1 to 371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2017-07-28"; volume: "10(1):344. doi"; pages: " 
            10.1186/s13104-017-2631-8"
COMMENT     Sequence Label: pBeloBac11loxP2272
FEATURES             Location/Qualifiers
     source          1..371
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(22..41)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(23..41)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(48..65)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(48..64)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(57..79)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(273..292)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgggtaccga gctcgaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt
       61 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
      121 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
      181 ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc
      241 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
      301 agccagcccc gacacccgcc aacacccgct gacgcgaacc ccttgcggcc gcatcgaata
      361 taacttcgta t
//
