LOCUS       sequence-121519-         599 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This is a BAC vector that contains both wt and mutant loxP sites.  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Coren JS
  TITLE     Retrofitting the BAC cloning vector pBeloBAC11 by the insertion of a
            mutant loxP site.
  JOURNAL   BMC Res Notes. 2017 Jul 28;10(1):344. doi: 
            10.1186/s13104-017-2631-8.
  PUBMED    28754135
REFERENCE   2  (bases 1 to 599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2017-07-28"; volume: "10(1):344. doi"; pages: " 
            10.1186/s13104-017-2631-8"
COMMENT     Sequence Label: pBeloBac11loxP2272
FEATURES             Location/Qualifiers
     source          1..599
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(28..46)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(29..46)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(64..80)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(64..80)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(77..99)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    88..104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(112..118,119..136,137..142))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    157..178
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    279..312
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 gacctgcagg catgcaagct tgagtattct atagtgtcac ctaaatagct tggcgtaatc
       61 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
      121 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
      181 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
      241 aatcggccaa cgcgaacccc ttgcggccgc ccctcgagat aacttcgtat aaagtatcct
      301 atacgaagtt atcccgggcc gtcgaccaat tctcatgttt gacagcttat catcgaattt
      361 ctgccattca tccgcttatt atcacttatt caggcgtagc aaccaggcgt ttaagggcac
      421 caataactgc cttaaaaaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat
      481 tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc
      541 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggc
//
