LOCUS       sequence-121523-        1029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: VIPR2-Tango
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctgg ctagcgttta aacttaagct tggtaccgag ctcggatcca
      121 ctagtccagt gtggtggaat tctgcagata tccagcacag tggcggccgc gccaccatga
      181 agacgatcat cgccctgagc tacatcttct gcctggtatt cgccgactac aaggacgatg
      241 atgacgccag catcgatatg cggacgctcc tgccacctgc cttgttgaca tgctggctct
      301 tggcgcctgt aaattcaatc caccccgagt gccgcttcca cctggagatc caggaggagg
      361 aaactaagtg cgccgaactc cttagaagcc agaccgagaa gcacaaggcc tgcagcggag
      421 tgtgggataa catcacctgt tggagaccag ccaacgtcgg ggagacagtt accgtgccat
      481 gtccaaaagt tttttctaac ttttactcta aagccgggaa tatctctaag aactgcacat
      541 ctgatggttg gagcgagaca tttccagact ttgtggatgc atgcgggtac agcgatccag
      601 aggatgagag caaaatcaca ttttatatcc ttgttaaagc catctacacc ctcggataca
      661 gcgttagtct gatgagtctg gccacaggca gtatcattct gtgcctcttt cggaaactgc
      721 actgcaccag gaactacatc catcttaatc tctttttgtc atttatcctg agggcaataa
      781 gcgtgttggt gaaggatgac gtgttgtatt ctagttccgg aaccctgcac tgtcccgatc
      841 agccatccag ttgggtaggg tgcaagctca gtctggtgtt cctgcaatat tgtattatgg
      901 ccaatttctt ctggctgttg gtggaggggc tctacctcca cactttgttg gtggctatgc
      961 tgccaccacg aaggtgcttt cttgcctacc tgctcattgg atggggcctt cctaccgtct
     1021 gcattgggg
//
