LOCUS       sequence-121533-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-terminal fusion to oxBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Costantini LM, Baloban M, Markwardt ML, Rizzo M, Guo F, Verkhusha 
            VV, Snapp EL
  TITLE     A palette of fluorescent proteins optimized for diverse cellular 
            environments.
  JOURNAL   Nat Commun. 2015 Jul 9;6:7670. doi: 10.1038/ncomms8670.
  PUBMED    26158227
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-07-9"; volume: "6:7670. doi"; pages: " 
            10.1038/ncomms8670"
COMMENT     Sequence Label: oxBFP
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(126..839,840..842))
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FISTTGKLPVPWPTLVTTLSHGVQVFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLLE
                     FRTAAGITLGMDELYK"
     primer_bind     779..800
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc tagagtcgcg
      121 gccgcttact tgtacagctc gtccatgccg agagtgatcc cggcggcggt gcggaactcc
      181 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagcacgga ctgggtgctc
      241 aggtagtggc tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag
      301 tggtcggcga gctgcacgct gccgtcctcc acgttgtggc ggatcttgaa gttggccttg
      361 atgccgttct tctgcttgac ggccatgata tagatgttgt ggctgttgaa gttgtactcc
      421 agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga cgcccttcag ctcgatgcgg
      481 ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtaggt gccgtcgtcc
      541 ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg
      601 tgctgcttca tgtggtcggg gtagcgggcg aacacctgca cgccgtggct cagggtggtc
      661 acgagggtgg gccagggcac gggcagcttg ccggtagtac tgatgaactt cagggtcagc
      721 ttgccgttgg tggcatcgcc ctcgccctcg cccctcacgc tgaacttgtg gccgtttacg
      781 tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc
      841 acc
//
