LOCUS       sequence-121561-        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  nesprin tension sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Arsenovic PT, Ramachandran I, Bathula K, Zhu R, Narang JD, Noll NA, 
            Lemmon CA, Gundersen GG, Conway DE
  TITLE     Nesprin-2G, a Component of the Nuclear LINC Complex, Is Subject to 
            Myosin-Dependent Tension.
  JOURNAL   Biophys J. 2016 Jan 5;110(1):34-43. doi: 10.1016/j.bpj.2015.11.014.
  PUBMED    26745407
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2015.11"; journalName: "Biophys J"; date: "2016-01-5-
            5"; volume: "110"; issue: "1"; pages: "34-43"
COMMENT     Sequence Label: pcdna nesprin TS
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(39..58)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             198..449
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     386..407
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
       61 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      121 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
      181 acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc
      241 acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
      301 gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc aaactgagca
      361 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
      421 tcactctcgg catggacgag ctgtacaagg cggccgcgca ggagcttcac agcaagctca
      481 ggctcaaaca gaccgtgcag cagctcaagt ctgacatcgg cagcatcgct gcttggcttg
      541 ggaaaactga ggccgagctg gaagctctga agttggccga gcctccctct gacatccagg
      601 aaatagcgct cagggtgaag agactgcaag agatactaaa agccttcgat acctacaagg
      661 ccttgatggt ctccgtcaat gtgagccaca aggaatatct gccaagccag agccccgaag
      721 ccacagaact ccaaaataga ctccatcagc tgagcctgtc ctgggactcg gtccagggcg
      781 tattggacag ctggcgagga gacttacggc agtcactcat gcagtgccag gactttcacc
      841 agctgagtca agacttgctc ctgtggctag cgacagcaga gagccgcagg cagaaggcgc
      901 atgtcaccag cccagaggcc gaccgccagg tcctcctgga gtgtcagaaa gacctaatgc
      961 gcctagaaaa ggagttggta gcccgtcagc ctcaagtcag ctccttg
//
