LOCUS       sequence-121562-        1136 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  nesprin headless control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Arsenovic PT, Ramachandran I, Bathula K, Zhu R, Narang JD, Noll NA, 
            Lemmon CA, Gundersen GG, Conway DE
  TITLE     Nesprin-2G, a Component of the Nuclear LINC Complex, Is Subject to 
            Myosin-Dependent Tension.
  JOURNAL   Biophys J. 2016 Jan 5;110(1):34-43. doi: 10.1016/j.bpj.2015.11.014.
  PUBMED    26745407
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2015.11"; journalName: "Biophys J"; date: "2016-01-5-
            5"; volume: "110"; issue: "1"; pages: "34-43"
COMMENT     Sequence Label: pcdna nesprin HL
FEATURES             Location/Qualifiers
     source          1..1136
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..736
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     primer_bind     complement(902..923)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gcgtttaaac gggccctcta gactcgagat ggtgagcaag ggcgaggaga ccacaatggg
       61 cgtaatcaag cccgacatga agatcaagct gaagatggag ggcaacgtga atggccacgc
      121 cttcgtgatc gagggcgagg gcgagggcaa gccctacgac ggcaccaaca ccatcaacct
      181 ggaggtgaag gagggagccc ccctgccctt ctcctacgac attctgacca ccgcgttcgc
      241 ctacggcaac agggccttca ccaagtaccc cgacgacatc cccaactact tcaagcagtc
      301 cttccccgag ggctactctt gggagcgcac catgaccttc gaggacaagg gcatcgtgaa
      361 ggtgaagtcc gacatctcca tggaggagga ctccttcatc tacgagatac acctcaaggg
      421 cgagaacttc ccccccaacg gccccgtgat gcagaagaag accaccggct gggacgcctc
      481 caccgagagg atgtacgtgc gcgacggcgt gctgaagggc gacgtcaagc acaagctgct
      541 gctggagggc ggcggccacc accgcgttga cttcaagacc atctacaggg ccaagaaggc
      601 ggtgaagctg cccgactatc actttgtgga ccaccgcatc gagatcctga accacgacaa
      661 ggactacaac aaggtgaccg tttacgagag cgccgtggcc cgcaactcca ccgacggcat
      721 ggacgagctg tacaaggggc caggtggtgc agggccaggt ggtgcagggc caggtggtgc
      781 agggccaggt ggtgcagggc ccggtggtgc aggtccaggt ggtgcaggtc caggtggtgc
      841 aggtccaggt ggtgctatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
      901 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
      961 gggcgatgcc acctacggca agctgaccct gaagctgatc tgcaccaccg gcaagctgcc
     1021 cgtgccctgg cccaccctcg tgaccaccct gggctacggc ctgcagtgct tcgcccgcta
     1081 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacg
//
