LOCUS       sequence-121565-       11355 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent fusion protein for tracking Akt signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11355)
  AUTHORS   Gross SM, Rotwein P
  TITLE     Akt signaling dynamics in individual cells.
  JOURNAL   J Cell Sci. 2015 Jun 3. pii: jcs.168773.
  PUBMED    26040286
REFERENCE   2  (bases 1 to 11355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2015 Jun 3. pii: jcs.168773."
COMMENT     Sequence Label: pLenti-FoxO1-Clover
FEATURES             Location/Qualifiers
     source          1..11355
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(132..200,201..992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(350..369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2075..2080,2081..2098,2099..2105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2118..2140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2137..2153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2172..2192
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             2448..2628
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2672..2797
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3290..3523
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3707..3751
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4012..4129
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4161..4185
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4298..5475
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4529..5466
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5423..5443
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     complement(7513..7534)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     8121..8142
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(8182..8206)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    8237..8825
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8290..8310)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8708..8719)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8839..9338
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8874..8897)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9271..9290
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9359..9958
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9359..9378)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9855..9875
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             10096..10329
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10401..10522
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10438..10457)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10492..10511
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10541..10676
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10708..10727)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10709..10727)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10737..10754)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10737..10753)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10746..10768)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10898..11353
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10985..11004)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11195..11216
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag
     4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta ttccagtgtg
     4141 gtggaattct gcagatatca acaagtttgt acaaaaaagc aggctttaaa ggaaccaatt
     4201 cagtcgacga taagctttgc aaagatggat aaagttttaa acagagagga atctttgcag
     4261 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
     4321 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
     4381 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     4441 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     4501 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
     4561 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccactggct gcagtacgtg
     4621 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
     4681 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
     4741 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
     4801 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
     4861 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
     4921 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
     4981 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc
     5041 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
     5101 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg
     5161 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
     5221 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg
     5281 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
     5341 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
     5401 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
     5461 tttcaggtgt cgtgaggaat ttcgacattt aaatttaatt aaaccatggc agaggctccc
     5521 caggtggtgg agaccgaccc ggacttcgag ccgctgcccc ggcagcgctc ctgtacctgg
     5581 ccgctgccca ggccggagtt taaccagtcc aactcgacca cctccagtcc ggcgccgtcg
     5641 ggcggcgcgg ccgccaaccc cgacgccgcg gcgagcctgg cctcggcgtc cgctgtcagc
     5701 accgacttta tgagcaacct gagcctgctg gaggagagtg aggacttcgc gcgggcgcca
     5761 ggctgcgtgg ccgtggcggc ggcggctgcg gccagcaggg gcctgtgcgg ggacttccag
     5821 ggccccgagg cgggctgcgt gcacccagcg ccgccacagc ccccaccgac cgggccgctg
     5881 tcgcagcccc cacccgtgcc tccctccgct gccgccgccg cggggccact cgcgggacag
     5941 ccgcgcaaga ccagctcgtc gcgccgcaac gcgtggggca acctgtcgta cgccgacctc
     6001 atcaccaagg ccatcgagag ctcagccgag aagaggctca ccctgtcgca gatctacgag
     6061 tggatggtga agagcgtgcc ctacttcaag gataagggcg acagcaacag ctcggcgggc
     6121 tggaagaatg caattcgccg caatctagcc cttcacagca agtttattcg agtgcagaat
     6181 gaaggaactg gaaagagttc ttggtggatg ctcaatccag agggaggcaa gagcggaaaa
     6241 tcaccccgga gaagagctgc gtccatggac aacaacagta aatttgctaa gagccgaggg
     6301 cgggctgcta agaaaaaagc atctctccgg tctgggcaag agggtcctgg agacagccct
     6361 gggtctcagt tttctaagtg gcctgcgagt cctgggtccc acagcaacga tgactttgat
     6421 aactggagta catttcgtcc tcgaaccagc tcaaatgcta gtaccatcag tgggagactt
     6481 tctcccatca tgacagagca ggatgacctg ggagatgggg acgtgcattc cctggtgtat
     6541 ccaccctctg ctgccaagat ggcgtctacg ctgcccagtc tgtctgaaat cagcaatcca
     6601 gaaaacatgg agaaccttct ggataatctc aaccttctct cgtccccaac atctttaact
     6661 gtgtccactc agtcctcgcc tggcagcatg atgcagcaga caccatgcta ttcgtttgca
     6721 ccgccaaaca ccagtctaaa ttcacccagt ccaaactact caaagtacac atacggccaa
     6781 tccagcatga gccctttgcc ccagatgcct atgcagacac ttcaggacag caaatcaagt
     6841 tacggaggat tgaaccagta taactgtgcc ccaggactct tgaaagagtt gttgacttct
     6901 gactctcctc cccacaatga cattatgtca ccggttgatc ccggagtggc ccaacccaac
     6961 agtcgggtcc tgggccaaaa tgtaatgatg ggccctaatt cggtcatgcc agcgtatggc
     7021 agccaggcat ctcataacaa aatgatgtac cccagctccc acacccaccc tggacatgca
     7081 cagcaaacgg cttcggtcaa cggccgtacc ctgccccatg tggtgaacac catgcctcac
     7141 acatctgcca tgaaccgctt gacccccgtg aagacacctt tacaagtgcc tctgtcccac
     7201 cccatgcaga tgagtgccct gggcaactac tcctcggtga gcagctgcaa tggctatggt
     7261 aggatgggtg tcctccacca ggagaagctc ccaagtgact tggatggcat gtttattgag
     7321 cgcttggact gtgacatgga gtccatcatt cggaatgacc ccatggatgg agataccttg
     7381 gattttaact ttgataatgt gttgcccaac caaagcttcc cacacagtgt caagactaca
     7441 acacacagct gggtgtcagg aggatctatg gtgagcaagg gcgaggagct gttcaccggg
     7501 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtccgc
     7561 ggcgagggcg agggcgatgc caccaacggc aagctgaccc tgaagttcat ctgcaccacc
     7621 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cgtggcctgc
     7681 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     7741 ggctacgtcc aggagcgcac catctctttc aaggacgacg gtacctacaa gacccgcgcc
     7801 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     7861 aaggaggacg gcaacatcct ggggcacaag ctggagtaca acttcaacag ccactacgtc
     7921 tatatcacag ccgacaagca gaagaacagc atcaaggcta acttcaagat ccgccacaac
     7981 gttgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     8041 ggccccgtgc tgctgcccga caaccactac ctgagccatc agtccgccct gagcaaagac
     8101 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgctgc cggaatcaca
     8161 ctgggatccc tcgaggtcta gacccagctt tcttgtacaa agtggttgat atccagcaca
     8221 gtggcggccg ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     8281 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     8341 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     8401 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     8461 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     8521 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     8581 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     8641 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     8701 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     8761 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     8821 cccgcctgga attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     8881 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     8941 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     9001 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     9061 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     9121 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     9181 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     9241 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     9301 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     9361 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     9421 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     9481 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     9541 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     9601 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     9661 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     9721 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     9781 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     9841 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     9901 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg
     9961 cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg catctcgagg
    10021 gcccggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
    10081 aagaaaaggg gggactggaa gggctagctc actcccaacg aagacaagat ctgctttttg
    10141 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctgcctag
    10201 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
    10261 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
    10321 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
    10381 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
    10441 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    10501 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
    10561 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
    10621 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
    10681 cttttgcgtc gagacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
    10741 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
    10801 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
    10861 tcccaacagt tgcgcagcct gaatggcgaa tggcgcgacg cgccctgtag cggcgcatta
    10921 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
    10981 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
    11041 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
    11101 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
    11161 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
    11221 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
    11281 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
    11341 acgtttacaa tttcc
//
