LOCUS       sequence-12157-e        7452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7452)
  AUTHORS   Yan Q, Bartz S, Mao M, Li L, Kaelin WG
  TITLE     The hypoxia-inducible factor 2alpha N-terminal and C-terminal 
            transactivation domains cooperate to promote renal tumorigenesis in 
            vivo.
  JOURNAL   Mol Cell Biol. 2007 Mar . 27(6):2092-102.
  PUBMED    17220275
REFERENCE   2  (bases 1 to 7452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2007 Mar . 27(6):2092-102."
COMMENT     Sequence Label: HA-HIF2-alpha P405A/N847A Delta-450-572-pBabe-Puro
FEATURES             Location/Qualifiers
     source          1..7452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             934..1350
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1318..1334
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1379..1405
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(3691..3711)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3696..4025
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3876..4011
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4035..4634
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(4035..4054)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4531..4551
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4733..5326
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5527..5544)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5698..6286)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5778..5797)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6464..7255,7256..7324))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7087..7106
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7325..7429)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt
      901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt
      961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc
     1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg
     1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca
     1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct
     1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt
     1261 ccatccgcgc cgtctctccc ccttgaacct cctctttcga ccccgcctca atcctccctt
     1321 tatccagccc tcactccttc tctaggcgcc ggccatcgag actaagctta ccatggccta
     1381 cccctatgat gttcccgatt atgctggatc cgccaccatg acagctgaca aggagaagaa
     1441 aaggagtagc tcggagagga ggaaggagaa gtcccgggat gctgcgcggt gccggcggag
     1501 caaggagacg gaggtgttct atgagctggc ccatgagctg cctctgcccc acagtgtgag
     1561 ctcccatctg gacaaggcct ccatcatgcg actggcaatc agcttcctgc gaacacacaa
     1621 gctcctctcc tcagtttgct ctgaaaacga gtccgaagcc gaagctgacc agcagatgga
     1681 caacttgtac ctgaaagcct tggagggttt cattgccgtg gtgacccaag atggcgacat
     1741 gatctttctg tcagaaaaca tcagcaagtt catgggactt acacaggtgg agctaacagg
     1801 acatagtatc tttgacttca ctcatccctg cgaccatgag gagattcgtg agaacctgag
     1861 tctcaaaaat ggctctggtt ttgggaaaaa aagcaaagac atgtccacag agcgggactt
     1921 cttcatgagg atgaagtgca cggtcaccaa cagaggccgt actgtcaacc tcaagtcagc
     1981 cacctggaag gtcttgcact gcacgggcca ggtgaaagtc tacaacaact gccctcctca
     2041 caatagtctg tgtggctaca aggagcccct gctgtcctgc ctcatcatca tgtgtgaacc
     2101 aatccagcac ccatcccaca tggacatccc cctggatagc aagaccttcc tgagccgcca
     2161 cagcatggac atgaagttca cctactgtga tgacagaatc acagaactga ttggttacca
     2221 ccctgaggag ctgcttggcc gctcagccta tgaattctac catgcgctag actccgagaa
     2281 catgaccaag agtcaccaga acttgtgcac caagggtcag gtagtaagtg gccagtaccg
     2341 gatgctcgca aagcatgggg gctacgtgtg gctggagacc caggggacgg tcatctacaa
     2401 ccctcgcaac ctgcagcccc agtgcatcat gtgtgtcaac tacgtcctga gtgagattga
     2461 gaagaatgac gtggtgttct ccatggacca gactgaatcc ctgttcaagc cccacctgat
     2521 ggccatgaac agcatctttg atagcagtgg caagggggct gtgtctgaga agagtaactt
     2581 cctattcacc aagctaaagg aggagcccga ggagctggcc cagctggctg ctaccccagg
     2641 agacgccatc atctctctgg atttcgggaa tcagaacttc gaggagtcct cagcctatgg
     2701 caaggccatc ctgcccccga gccagccatg ggccacggag ttgaggagcc acagcaccca
     2761 gagcccactg gcccctgtag ccccgcacag tcccttcctc ctggacaagt ttcagcagca
     2821 gctggagagc aagaagacag agcccgagca ccggcccatg tcctccatct tctttgatgc
     2881 cggaagcaaa gcatccctgc caccgtgctg tggccaggcc agcacccctc tctcttccat
     2941 ggggggcaga tccaataccc agtggccccc agatccacca ttacattttg ggcccacaaa
     3001 gtgggccgtc ggggatcagc gcacagagtt cttgggagca gcgccgttgg ggccccctgt
     3061 ctctccaccc catgtctcca ccttcaagac aaggtctgca aagggttttg gggctcgagg
     3121 cccagacgtg ctgagtccgg ccatggtagc cctctccaac aagctgaagc tgaagcgaca
     3181 gctggagtat gaagagcaag ccttccagga cctgagcggg ggggacccac ctggtggcag
     3241 cacctcacat ttgatgtgga aacggatgaa gaacctcagg ggtgggagct gccctttgat
     3301 gccggacaag ccactgagcg caaatgtacc caatgataag ttcacccaaa accccatgag
     3361 gggcctgggc catcccctga gacatctgcc gctgccacag cctccatctg ccatcagtcc
     3421 cggggagaac agcaagagca ggttcccccc acagtgctac gccacccagt accaggacta
     3481 cagcctgtcg tcagcccaca aggtgtcagg catggcaagc cggctgctcg ggccctcatt
     3541 tgagtcctac ctgctgcccg aactgaccag atatgactgt gaggtggccg tgcccgtgct
     3601 gggaagctcc acgctcctgc aaggagggga cctcctcaga gccctggacc aggccacctg
     3661 acaattcgcc agcacagtgg tcgaccctgt ggaatgtgtg tcagttaggg tgtggaaagt
     3721 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3781 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3841 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     3901 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3961 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     4021 gcaaaaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     4081 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     4141 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     4201 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     4261 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     4321 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     4381 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     4441 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     4501 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     4561 tcaccgtcac cgccgacgtc gagtgcccga aggaccgcgc gacctggtgc atgacccgca
     4621 agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac
     4681 cccatgcatc gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag
     4741 accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa
     4801 atacataact gagaatagag aagttcagat caaggtcagg aacagatgga acagctgaat
     4861 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
     4921 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
     4981 cagggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagagaacc
     5041 atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa
     5101 ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga
     5161 gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg
     5221 tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag
     5281 ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatttccg acttgtggtc
     5341 tcgctgcctt gggagggtct cctctgagtg attgactacc cgtcagcggg ggtcttcaca
     5401 tgcagcatgt atcaaaatta atttggtttt ttttcttaag tatttacatt aaatggccat
     5461 agttgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt ggcgctcttc
     5521 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     5581 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     5641 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     5701 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     5761 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     5821 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     5881 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     5941 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     6001 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     6061 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     6121 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     6181 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     6241 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     6301 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     6361 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gtttgcggcc
     6421 gcaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     6481 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     6541 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     6601 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     6661 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     6721 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     6781 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     6841 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     6901 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     6961 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7021 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     7081 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     7141 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7201 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     7261 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     7321 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     7381 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     7441 gaaaagtgcc ac
//
