LOCUS       sequence-121581-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This G protein alpha-o construct contains internal insertions of YFP
            and the EE epitope.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Yost EA, Mervine SM, Sabo JL, Hynes TR, Berlot CH
  TITLE     Live cell analysis of G protein beta5 complex formation, function, 
            and targeting.
  JOURNAL   Mol Pharmacol. 2007 Oct;72(4):812-25. Epub 2007 Jun 27.
  PUBMED    17596375
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2007-10"; volume: "72(4)"; pages: "812-25. Epub 
            2007 Jun 27"
COMMENT     Sequence Label: G protein alpha-o-YFP
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     204..225
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      645..654
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca
       61 catcaggccg tagccgaagg tggtcacgag ggtgggccag ggcacgggca gcttgccggt
      121 ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga
      181 cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc
      241 ggtgaacagc tcctcgccct tgctcaccat gctcccgccc ccaccggatc ttggttcagt
      301 gtcctccatg cgactcacca cgtcacacac catcttggag tctgccttcc tctccttgtc
      361 accatactcc acgcccagag tatccatggc ccgcacaatg gctgccagag actggatggt
      421 gttgctgtag acgacaggct tgtactgctt tacgtcttct ccagagaagc catcttcatg
      481 gatgatcttc atctgcttca caatggtgct ttttcctgat tctccagccc ccagcaggag
      541 taatttcacg tctttggcgg cgctgatgcc atcttctttg agatttttct caatcgcctt
      601 gctccgctcg agggcggctc tctcctctgc gctcagagta catcccatgg tggccccttc
      661 cctgccacag cccgcacgac tcggctggca cgactcctcg ggtcggcgcg ccccccaccc
      721 ccccacagag cagagcctgg gctcaaaggg gagaaaatca cgatatcctt tccttggttg
      781 ggaacaaa
//
