LOCUS       sequence-121585-         508 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This G protein alpha-o construct contains internal insertions of YFP
            and the EE epitope.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Yost EA, Mervine SM, Sabo JL, Hynes TR, Berlot CH
  TITLE     Live cell analysis of G protein beta5 complex formation, function, 
            and targeting.
  JOURNAL   Mol Pharmacol. 2007 Oct;72(4):812-25. Epub 2007 Jun 27.
  PUBMED    17596375
REFERENCE   2  (bases 1 to 508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2007-10"; volume: "72(4)"; pages: "812-25. Epub 
            2007 Jun 27"
COMMENT     Sequence Label: G protein alpha-o-YFP
FEATURES             Location/Qualifiers
     source          1..508
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..293
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     230..251
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
       61 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
      121 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
      181 tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
      241 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtccggag
      301 gtggaggatc tttctctgca gaactgcttt ctgccatgat gcgactctgg ggcgactcgg
      361 ggatccagga gtgcttcaac cgatctcggg agtatcagct caacgactct gccaaatact
      421 acctggacag cttggatcgg attggagctg cagaatacat gcccaccgag caggacatcc
      481 tccgaaccag ggtcaaaaca actggcat
//
