LOCUS       sequence-121589-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express FLAG-tagged C.elegans WTS-1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Iwasa H, Maimaiti S, Kuroyanagi H, Kawano S, Inami K, Timalsina S, 
            Ikeda M, Nakagawa K, Hata Y
  TITLE     Yes-associated protein homolog, YAP-1, is involved in the 
            thermotolerance and aging in the nematode Caenorhabditis elegans.
  JOURNAL   Exp Cell Res. 2013 Apr 15;319(7):931-45. doi: 
            10.1016/j.yexcr.2013.01.020. Epub 2013 Feb 7.
  PUBMED    23396260
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.yexcr.2013.01.020"; journalName: "Exp Cell Res"; date: 
            "2013-04-15- 15"; volume: "319"; issue: "7"; pages: "931-45"
COMMENT     Sequence Label: pFLAG-WTS-1
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..65
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     CDS             66..89
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 accgtcagaa ttaattcacc atgtctgcac ttctgatcct agctcttgtt ggagctgcag
       61 ttgctgacta caaagacgat gacgacaagc ttgcggccgc gcgacctgct gcccctggta
      121 ctactccaaa tggtgcatct tctgatatca gacatcaacg tggagttgct ccgatcccat
      181 ttggctctac taattcagcg atagatgccc atcacaattc agaaatccgc gtggggcgtc
      241 atcgtgcaaa actcgacgaa attcgcgaat cgctgaaagc ttacgaacat gaagccggtt
      301 tattatcaag tcacgtggct cttggatcac ttgcaacacc gtcaagttct tccgtcagcc
      361 attctgacat caccaatgac aatgcggaag tgatgaattt ttcgtcttct tcttcaaatg
      421 cagcagcgac gacgacgaca gtctcgagcg ctgctgtatc aaattcgaat tcttttagaa
      481 cagaaggtgg tggtcacaag atgcggataa caccaatgcc acaaagacat ttaatgatgg
      541 atactggtgc caatgggaca gtatttcgaa gtggaaaaga gatgattcga aatggaaatc
      601 catcaacaac tatatcatca acaccttcta caacaactga agagtcgatt aggatacatc
      661 ctgctggata tcgatatgat atgccaactc ctgcatatca tatgaataac aatgcaccac
      721 aatactcacc aggatattct cgaccacctc caccagccta cgattcatct ccggtgaaca
      781 ctagaatgac tccagttgca acagacaact accgtactca cctgcatatg aaagttcatc
      841 ctgttgta
//
