LOCUS       sequence-121594-         538 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses dsRed and shRNA to knock down adra2a receptors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Zhang Z, Ferretti V, Guntan I, Moro A, Steinberg EA, Ye Z, Zecharia 
            AY, Yu X, Vyssotski AL, Brickley SG, Yustos R, Pillidge ZE, Harding 
            EC, Wisden W, Franks NP
  TITLE     Neuronal ensembles sufficient for recovery sleep and the sedative 
            actions of alpha2 adrenergic agonists.
  JOURNAL   Nat Neurosci. 2015 Apr;18(4):553-61. doi: 10.1038/nn.3957. Epub 2015
            Feb 23.
  PUBMED    25706476
REFERENCE   2  (bases 1 to 538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.3957"; 
            journalName: "Nat Neurosci"; date: "2015-04"; volume: "18"; issue: 
            "4"; pages: "553-61"
COMMENT     Sequence Label: AAV-actin prom-dsRed-adra2a.1227.shRNA
FEATURES             Location/Qualifiers
     source          1..538
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    82..115
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(259..277)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(309..329)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 gtatcctata cgaagttata tcaaaatagg aagaccaatg cttcaccatc gacccgaatt
       61 gccaagcatc accatcgacc cataacttcg tataatgtat gctatacgaa gttatgaatt
      121 cctgcaggat ggactcctcc gaggacgtca tcaaggagtt catgcgcttc aaggtgcgca
      181 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
      241 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct
      301 gggacatcct gtccccccag ttccagtacg gctccaaggt gtacgtgaag caccccgccg
      361 acatccccga ctacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
      421 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccttacag gacggctcct
      481 tcatctacaa ggtgaagttc atcggcgtga acttcccctc cgacggcccc gtaatgca
//
