LOCUS       sequence-121595-         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  miniSOG fused to N terminal 29 aa of human Cox8a for targeting to 
            the mitochondria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Qi YB, Garren EJ, Shu X, Tsien RY, Jin Y
  TITLE     Photo-inducible cell ablation in Caenorhabditis elegans using the 
            genetically encoded singlet oxygen generating protein miniSOG.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 May 8;109(19):7499-504. doi: 
            10.1073/pnas.1204096109. Epub 2012 Apr 24.
  PUBMED    22532663
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1204096109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-05-8- 8"; volume: "109"; issue: "19"; pages: "7499-504"
COMMENT     Sequence Label: pCZGY1026 COX8aN::miniSOG in pENTR[1-2]
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    61..85
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             86..160
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      188..197
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             194..514
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     protein_bind    complement(515..614)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(631..650)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(632..650)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(655..671)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(655..671)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt ttttataatg
       61 ccaactttgt acaaaaaagc aggctatgtc cgtcctgacg ccgctgctgc tgcggggctt
      121 gacaggctcg gcccggcggc tcccagtgcc gcgcgccaag atccattcgt tgggggatcc
      181 accggtcgcc accatggaga aaagtttcgt gataactgat ccacggctgc cagacaatcc
      241 catcatcttc gcatccgatg gcttcctgga gctgaccgag tattccagag aggagatcct
      301 gggccgcaat ggccgctttc tgcagggacc agagacagac caggccacag tgcagaagat
      361 tcgcgatgcc attagagatc agcgcgagat taccgtgcag ctgataaact acacaaaaag
      421 cgggaagaaa ttctggaacc tcctgcacct ccagcccatg agggaccaga agggtgagct
      481 ccagtatttc atcggagtgc agctggatgg ataaacccag ctttcttgta caaagttggc
      541 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
      601 aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata
      661 gctgtttcct ggcagctctg gcccgtgtct caa
//
