LOCUS       sequence-121597-         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  miniSOG fused to N terminal 55 aa of C. elegans tomm-20 for 
            targeting to the mitochondria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Qi YB, Garren EJ, Shu X, Tsien RY, Jin Y
  TITLE     Photo-inducible cell ablation in Caenorhabditis elegans using the 
            genetically encoded singlet oxygen generating protein miniSOG.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 May 8;109(19):7499-504. doi: 
            10.1073/pnas.1204096109. Epub 2012 Apr 24.
  PUBMED    22532663
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1204096109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-05-8- 8"; volume: "109"; issue: "19"; pages: "7499-504"
COMMENT     Sequence Label: pCZGY1534 tomm-20N::miniSOG in pENTR[1-2]
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    59..83
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             255..569
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="EKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQGP
                     ETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQLD
                     G"
     protein_bind    complement(585..684)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(701..720)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(702..720)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(725..741)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(725..741)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt ttataatgcc
       61 aactttgtac aaaaaagcag gctatgtcgg acacaattct tggtttcaac aaatcaaacg
      121 tcgttttggc tgctggaatt gctggagccg ctttcctcgg ctactgcatt tacttcgatc
      181 ataagagaat caacgctcca gactacaagg acaagattag gcaaaagaga cgtgcccagg
      241 ctggagcatc cggagagaaa agtttcgtga taactgatcc acggctgcca gacaatccca
      301 tcatcttcgc atccgatggc ttcctggagc tgaccgagta ttccagagag gagatcctgg
      361 gccgcaatgg ccgctttctg cagggaccag agacagacca ggccacagtg cagaagattc
      421 gcgatgccat tagagatcag cgcgagatta ccgtgcagct gataaactac acaaaaagcg
      481 ggaagaaatt ctggaacctc ctgcacctcc agcccatgag ggaccagaag ggtgagctcc
      541 agtatttcat cggagtgcag ctggatggag gatcttccgg ataaacccag ctttcttgta
      601 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
      661 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta
      721 catggtcata gctgtttcct ggcagctctg gcccgtgtct caa
//
